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Table 1 Identities of HT induced/decreased proteins in the peels of Satsuma mandarin fruit

From: Comparative proteomic and metabolomic profiling of citrus fruit with enhancement of disease resistance by postharvest heat treatment

Glycolysis and TCA cycle: 9

No.

Protein name

Protein accumulation

Source organism

GI no.

TheoMr/pI

Expt Mr/pI

PC

MS

H1

Enolase

Glycine max

42521309

47.97/5.31

50.84/5.23

8

443

H2

Phosphoglycerate kinase, putative

Arabidopsis thaliana

15223484

50.02/8.27

41.88/4.90

9

108

H3

Putative 2-oxoglutarate dehydrogenase E2 subunit

Oryza sativa

48716382

49.46/6.78

43.64/4.62

4

105

H4

Malate dehydrogenase, cytoplasmic

Beta vulgaris

11133601

35.81/5.89

37.61/4.61

7

347

H5

Triosephosphate isomerase, cytosolic (TIM)

Zea mays

136063

27.23/5.52

24.97/4.47

9

310

H6

Malate dehydrogenase, mitochondrial precursor

Citrullus lanatus

126896

36.40/8.88

36.36/4.71

8

376

H7

Pyruvate dehydrogenase E1 beta subunit isoform 2

Zea mays

162458637

40.23/5.56

35.91/5.72

5

302

H8

NAD-malate dehydrogenase

Nicotiana tabacum

5123836

43.67/8.03

34.10/5.38

11

436

H9

Putative2,3-bisphosphoglycerate- independent phosphoglycerate mutase

Arabidopsis thaliana

15982735

60.67/5.27

61.18/5.12

9

207

Redox regulation: 9

No.

Protein name

Protein accumulation

Source organism

GI no.

TheoMr/pI

Expt Mr/pI

PC

MS

H10

Isoflavone reductase related protein

Pyrus communis

3243234

33.80/6.02

33.12/4.53

5

92

H11

Oxidoreductase, zinc-binding dehydrogenase family protein

Arabidopsis thaliana

15220854

41.13/8.46

37.58/5.41

6

93

H12

Putative NAD(P)H oxidoreductase, isoflavone reductase

Arabidopsis thaliana

19310585

34.24/6.61

34.43/4.71

4

67

H13

Aldo/keto reductase family protein

Arabidopsis thaliana

42571931

27.76/6.33

38.36/5.16

6

164

H14

2-oxoacid dehydrogenase family protein

Arabidopsis thaliana

15240454

50.27/9.19

47.44/4.92

7

168

H15

Mitochondrial processing peptidase

Solanum tuberosum

587562

54.62/5.99

49.29/5.42

4

65

H16

Copper/zinc superoxide dismutase

Citrus limon

33340236

15.20/5.46

17.02/5.27

4

263

H17

Putative NAD(P)H oxidoreductase, isoflavone reductase

Arabidopsis thaliana

19310585

34.24/6.61

34.47/4.92

3

85

H18

Chloroplast stromal ascorbate peroxidase

Vigna unguiculata

45268439

39.95/7.06

31.00/5.06

9

191

Stress response: 7

No.

Protein name

Protein accumulation

Source organism

GI no.

TheoMr/pI

Expt Mr/pI

PC

MS

H19

Abscisic stress ripening-like protein

Prunus persica

16588758

20.74/5.68

29.89/5.25

4

316

H20

Class III chitinase

Benincasa hispida

5919201

33.03/9.8

26.84/6.32

3

65

H21

Heat shock protein 60

Prunus dulcis

24637539

58.06/5.26

88.79/5.66

10

205

H22

17.7 kDa heat shock protein

Carica papaya

37933812

17.77/6.39

19.12/5.95

2

98

H23

Putative heat shock 70 KD protein, mitochondrial precursor

Oryza sativa

27476086

70.68/5.45

92.99/5.65

17

358

H24

Low molecular weight heat-shock protein

Pseudotsuga menziesii

1213116

18.18/5.82

17.78/6.39

4

122

H25

Beta-1, 3-glucanase

Citrus sinensis

2274915

37.32/9.19

34.38/6.18

7

98

Other metabolism: 6

No.

Protein name

Protein accumulation

Source organism

GI no.

TheoMr/pI

Expt Mr/pI

PC

MS

H26

Fiber annexin

Gossypium hirsutum

3493172

36.20/6.34

36.23/5.53

5

70

H27

Alpha-amylase

Citrus reticulata

20336385

17.05/4.91

44.16/5.64

8

177

H28

Glutamine synthetase

Elaeagnus umbellata

47933888

39.33/6.10

39.38/5.16

5

134

H29

Putative inorganic pyrophosphatase

Oryza sativa

46805453

20.45/4.61

28.58/6.31

4

74

H30

Thiazole biosynthetic enzyme, chloroplast precursor

Citrus sinensis

6094476

37.74/5.40

31.35/6.04

12

447

H31

Lipocalin protein

Capsicum annuum

50236424

21.41/7.66

19.0/4.33

3

143

Protein folding: 6

No.

Protein name

Protein accumulation

Source organism

GI no.

TheoMr/pI

Expt Mr/pI

PC

MS

H32

Catalytic/coenzyme binding

Arabidopsis thaliana

18404496

34.97/8.37

25.54/4.50

6

252

H33

Chaperonin-60 beta subunit

Solanum tuberosum

1762130

63.26/5.72

57.6/5.77

12

283

H34

Protein disulfide isomerase

Zea mays]

162461925

40.43/6.29

37.7/5.26

9

165

H35

Transcription factor APFI

Arabidopsis thaliana

13507025

30.14/6.24

28.15/5.14

3

89

H36

Putative ATP synthase beta subunit

Oryza sativa

56784991

45.93/5.33

51.13/5.66

20

695

H37

Putative ATP synthase beta subunit

Oryza sativa

56784991

45.93/5.33

50.0/5.6

20

808

  1. Samples at 1, 6, 12 and 32 DAT were used for differential analysis of proteins accumulation. After 2-DE, protein spot intensities were quantified using PDQuest 2-D software version 7.4. Comparative and statistical analysis shows there were only 50 differentially displayed protein spots whose abundance was altered at least 1.5-fold between the HT and control during the same time period. Of these, 34 proteins were successfully identified using Applied Biosystems 4800 matrix-assisted laser desorption/ionization-time-of-flight tandem mass spectrometry (MALDI-TOF MS) analysis. Protein relative accumulation is represented by the column configuration, and accumulation at 1, 6, 16 and 32 DAT is shown from left to right. Protein name and GI No. are from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/guide/). Experimental molecular mass and pI (Exp. M/pI) were calculated using PDQuest software (version 7.4), and mass is shown in kDa. PC: Peptide count. MS: Mascot score.