Figure 2
Evidence for dimerization by the GmRLK18-1 LRR-domain. Panel (A) 12 % (w/v) non-denatured PAGE of; lane 1 BenchmarkTM prestained protein ladder; lane 2, purified GmRLK18-1-LRR. Refolded GmRLK18-1-LRR showed presence of monomer as well as homodimer. Loading on SDS PAGE under reduced conditions showed only a single band [6]. Panel (B); A 12 % (w/v) non-denatured PAGE of; lane 1 BenchmarkTM prestained protein ladder; lane 2, GmRLK18-1 proteins detected by the anti-GmRLK18-1 antibody. Proteins were isolated from roots and refolded GmRLK18-1 showed presence of monomers as well as complexes in the correct position to be homo-dimers.