Figure 1From: The ArabidopsisAt1g30680 gene encodes a homologue to the phage T7 gp4 protein that has both DNA primase and DNA helicase activitiesPurification of the recombinant protein. Panel A shows the Coomassie Blue-stained gel, with increasing amounts of the purified recombinant (lanes 3, 5 and 7) and control (lanes 2, 4 and 6) protein, from left to right. Lane 1, protein molecular weight markers (Invitrogen SeeBlue 2 markers). Lanes 2 and 3, 0.195Â ng; lanes 4 and 5, 0.39Â ng; lanes 6 and 7, 0.585Â ng. Panel B shows a western blot of the purified protein using antibody against the Arabidopsis Twinkle homologue. Lane 1 contains molecular weight markers (Invitrogen Magic Markers). Lane 2, control protein; lane 3, 0.5Â ng purified recombinant protein. The arrow at the right indicates 80Â kDa, the length of the full-length Arabidopsis gene product. The recombinant protein is slightly smaller (~74Â kDa) as it lacks the N-terminal localization sequence.Back to article page