Partial functional conservation of IRX10 homologs in physcomitrella patens and Arabidopsis thalianaindicates an evolutionary step contributing to vascular formation in land plants

Table 2 Phobius transmembrane and signal sequence prediction for members of the Arabidopsis , Populus and Physcomitrella IRX10 family of proteins

Protein	Amino acid	Prediction	Protein	Amino acid	Prediction
AtIRX10	1-21	Signal	AtIRX10-L	1-19	Signal
	1-5	N-region		1-2	N-region
	6-16	H-region		3-13	H-region
	17-21	C-region		14-21	C-region
	22-412	Non-cytoplasmic		22-415	Non-cytoplasmic
PtGT47A-1	1-19	Signal	PtGT47A-2	1-19	Signal
	1-2	N-region		1-2	N-region
	3-14	H-region		3-14	H-region
	15-19	C-region		15-19	C-region
	20-412	Non-cytoplasmic		20-413	Non-cytoplasmic
PtGT47D-1 and PtGT47D-4	1-5	Cytoplasmic	PpGT47A	1-31	Cytoplasmic
	6-26	Transmembrane		32-51	Transmembrane
	27-417	Cytoplasmic		52-449	Non-cytoplasmic

IRX10 related protein sequences from Arabidopsis, Populus and Physcomitrella were analysed using the Phobius programme (http://www.phobius.sbc.su.se) for the presence of N-terminal signal sequences and the locations of the hydrophobic predicted transmembrane core (H-region) flanked by the N- and C- regions.

ISSN: 1471-2229