Figure 3
Comparison of GC/MS-determined 1,8-cineole levels to cineole synthase gene expression profiles generated by DNA microarray analysis. 1,8-Cineole levels in either turmeric or ginger are shown as bar graphs. Gene expression levels from normalized microarray spot intensities are shown as the line graph. Error bars indicate standard errors of the mean. The sequence for 1,8-cineole synthase used in this comparison was identified from ginger rhizomes. Both metabolite profiling and gene expression profiling of the ginger 1,8-cineole synthase match very well for ginger. However, 1,8-cineole produced in turmeric appears to be produced by an enzyme that is not the same as that from ginger. Bottom axis abbreviations represent developmental time courses for three cultivars, G: white ginger, F: turmeric variety FMO (Fat Mild Orange), and T: turmeric variety TYA (Thin Yellow Aromatic), respectively. Rh: rhizome, R: root, L: leaf. 2Â M, 3Â M, 4Â M, 5Â M, 7Â M represent plants of different ages in months, e.g., 2Â M: 2Â months, 3Â M: 3Â months, etc.