Large-scale proteomic analysis of the grapevine leaf apoplastic fluid reveals mainly stress-related proteins and cell wall modifying enzymes

Delaunois, Bertrand; Colby, Thomas; Belloy, Nicolas; Conreux, Alexandra; Harzen, Anne; Baillieul, Fabienne; Clément, Christophe; Schmidt, Jürgen; Jeandet, Philippe; Cordelier, Sylvain

doi:10.1186/1471-2229-13-24

Table 1 Annotation of the proteins in the grapevine apoplastic fluid leaf

From: Large-scale proteomic analysis of the grapevine leaf apoplastic fluid reveals mainly stress-related proteins and cell wall modifying enzymes

Classes^a	Subclasses^b	Spots n°^c	Gi acc. n°^d	Loc.^e	Closer homologous protein by Blastp [species]^f	% hom.^g
PR proteins	chitinases	19;55;127;134;135	157329111	C	chitinase, putative [Ricinus communis]	75
		31	15213852	S	chitinase-B [Sorghum halepense]	100
		44	225462669	S	class III chitinase [Vitis vinifera]	99
		55;59;121;122;125;127;128;134;135	225469348	S	chitinase, putative [Ricinus communis]	77
		65	10880381	S	chitinase [Vitis vinifera]	100
		140;141;175	116333	S	chitinase B [Zea mays]	100
		142	147820457	S	chitinase 1 precursor, putative [Ricinus communis]	76
		144	266324	NC	chitinase-B [Sorghum halepense]	62
		147;148;149	225454408	S	acidic chitinase III [Nicotiana tabacum]	82
		155	225434076	C	class IV chitinase [Vitis pseudoreticulata]	87
		176	157353734	C	class IV chitinase [Vitis pseudoreticulata]	87
	glucanases	136	163914215	S, TM	beta 1–3 glucanase [Vitis hybrid cultivar]	100
		39;40;45;46;47;63;136	225441375	S, TM	beta 1–3 glucanase [Vitis vinifera]	99
		24;41;42;61;64;136	225441379	S	beta-1,3-glucanase [Vitis riparia]	99
		27;167;168;169	225441373	S, TM	beta-1,3-glucanase [Vitis riparia]	86
		22	170243	S, TM	beta-1,3-glucanase [Vitis vinifera]	84
	other PR proteins	35	225429115	S	pathogenesis-related protein 1 [Vitis hybrid cultivar]	100
		36	225429250	S	pathogenesis-related protein 1 [Vitis hybrid cultivar]	88
		37	225429119	S	putative pathogenesis-related protein 1 [Vitis hybrid cultivar]	98
		33;34;36;49;160;161;163;173	225426801	S, TM	osmotin-like protein [Vitis vinifera]	90
		177	7406714	M	putative thaumatin-like protein [Vitis vinifera]	100
		162;177	225426793	S, TM	thaumatin-like protein [Vitis vinifera]	99
		145;146;150;151;156	225444754	S	germin-like protein 6 [Vitis vinifera]	100
		152;153;154;157;159	225429295	S	NtPRp27 [Nicotiana tabacum]	83
		166	147853970	UC	pathogenesis-related protein 10.7 [Vitis vinifera]	73
		164	225453020	S	Wound-induced protein WIN2 precursor, putative [Ricinus communis]	82
proteases	serine proteinases	83	225449346	NC	cucumisin precursor, putative [Ricinus communis]	76
		3;80;81;82;83;89;90;94;95;119	157344189	NC	cucumisin precursor, putative [Ricinus communis]	78
		68;70;71;72	157335112	NC	subtilisin-type protease precursor [Glycine max]	80
		69	1771160	S	xylem serine proteinase 1 precursor, putative [Ricinus communis]	85
		110	157335622	NC	xylem serine proteinase 1 precursor, putative [Ricinus communis]	85
		4;5	157345245	S, TM	xylem serine proteinase 1 precursor, putative [Ricinus communis]	89
		111	225457767	S	serine carboxypeptidase, putative [Ricinus communis]	88
		172	157348245	S, TM	serine carboxypeptidase, putative [Ricinus communis]	79
	aspartic proteinases	48;115;117;118;119;123;126	157335788	M	aspartic proteinase nepenthesin-1 precursor, putative [Ricinus communis]	85
		14;18;129	157339844	S	aspartic proteinase nepenthesin-2 precursor, putative [Ricinus communis]	75
		15;16;17;57;99;102;103;120;124	225430555	S	aspartic proteinase nepenthesin-2 precursor, putative [Ricinus communis]	76
cell wall metabolism	hydrolases	28;29;171	147771556	S, TM	xyloglucan endotransglucosylase/hydrolase [Gossypium hirsutum]	90
		67	157354845	S, TM	glycosyl hydrolase family 38 protein [Arabidopsis thaliana]	77
	glucosidases	1	147821903	S, TM	alpha-glucosidase, putative [Ricinus communis]	87
		66	147787240	S	alpha-glucosidase, putative [Ricinus communis]	79
		2;77;78;79;107;108;109	225423961	S, TM	alpha-glucosidase, putative [Ricinus communis]	90
		84;85	157350003	S	beta-glucosidase, putative [Ricinus communis]	90
		84;85;86;87;88;96	157355824	S, TM	beta-glucosidase, putative [Ricinus communis]	90
	galactosidases	101	147810287	UC	alpha-galactosidase/alpha-n-acetylgalactosaminidase, putative [Ricinus communis]	92
		58	157329180	NC	beta-galactosidase, putative [Ricinus communis]	87
		13;50;53;56;98;174	157337481	S, TM	beta-galactosidase, putative [Ricinus communis]	89
		97;112	157332401	S	beta-galactosidase, putative [Ricinus communis]	88
	fucosidase	15;53;54	225424647	S, TM	alpha-L-fucosidase 2 precursor, putative [Ricinus communis]	82
	arabinofuranosidase	3;6;7	225440254	S, TM	alpha-N-arabinofuranosidase 1 precursor, putative [Ricinus communis]	83
	polygalacturonase	81	225426168	S, TM	polygalacturonase, putative [Ricinus communis]	88
	pectinesterases	43;49	157353359	NC	pectinesterase-2 precursor, putative [Ricinus communis]	71
		56;130	157350105	S, TM	pectinesterase-3 precursor, putative [Ricinus communis]	78
		73	157353908	S	pectin methylesterase 2 [Pyrus communis]	86
peroxidases		23;26;29;62	225459180	S	cationic peroxidase [Arachis hypogaea]	85
		60	157355449	S, TM	class III peroxidase [Gossypium hirsutum]	86
		21;25	157355447	S, TM	class III peroxidase [Gossypium hirsutum]	89
		20;75;76;105;106	225435616	S	peroxidase [Armoracia rusticana]	80
		131;158	223635592	NC	peroxidase 1 [Scutellaria baicalensis]	80
		131	157342951	S	peroxidase 25 precursor, putative [Ricinus communis]	83
other stress related proteins		165	134684	C	putative Cu/Zn superoxide dismutase precursor [Vitis vinifera]	83
		127	26224736	NC	serpin-like protein [Citrus x paradisi]	85
		113;114	147846526	S	reticuline oxidase precursor, putative [Ricinus communis]	80
		132;133	147825300	S, TM	reticuline oxidase precursor, putative [Ricinus communis]	82
		21	76559894	UC	isoflavone reductase-like protein 5 [Vitis vinifera]	100
		10;11;12	157360089	S	heparanase, putative [Ricinus communis]	84
		43	115488670	C	universal stress protein family protein, expressed [Oryza sativa Japonica Group]	100
micellaneous		138;139;170	225443264	S	acid phosphatase [Glycine max]	79
		104	147814943	S, TM	alpha-amylase [Vigna angularis]	81
		90	91983977	UC	ATP synthase CF1 alpha subunit [Vitis vinifera]	100
		81	224365667	UC	ATPase alpha subunit [Vitis vinifera]	100
		137	157343592	UC	chloroplast heat shock protein 70–2 [Ipomoea nil]	93
		52	225466690	C	chloroplast sedoheptulose-1,7-bisphosphatase [Solanum lycopersicum]	95
		32	225457957	UC	cyclophilin [Catharanthus roseus]	94
		124	225454430	S, TM	lanatoside 15′-O-acetylesterase [Digitalis lanata]	84
		83	157343878	S	lysosomal alpha-mannosidase, putative [Ricinus communis]	86
		20;92;93	157343877	UC	lysosomal alpha-mannosidase, putative [Ricinus communis]	83
		51;52;74	157341194	S	lysosomal alpha-mannosidase, putative [Ricinus communis]	87
		143	225440390	NC	NAD-dependent epimerase/dehydratase [Zea mays]	92
		32;38	225453348	UC	nucleoside diphosphate kinase B [Jatropha curcas]	95
		119	147769722	C	polyprotein [Oryza australiensis]	58
		100	11385598	M	putative chloroplast RNA helicase VDL’ isoform 2 [Nicotiana tabacum]	100
		8	149774708	NC	ribulose-1,5-bisphosphate carboxylase/oxygenase [Ophioglossum petiolatum]	100
		9	147769051	UC	ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Vitis vinifera]	94
		116	225436591	UC	serine-pyruvate aminotransferase, putative [Ricinus communis]	95
		19	116830469	C	hypothetical protein [Arabidopsis thaliana]	100
		30	125563554	UC	hypothetical protein [Vitis vinifera]	49
		69	157343556	C	hypothetical protein [Vitis vinifera]	100
		91	147818959	NC	Ca/calmodulin-dependent protein kinase	40

^a the protein distribution according to their biological process.
^b the protein group.
^c the number that identifies protein spots on 2-D apoplastic map.
^d the accession number from GenBank assigned to the peptide after MS/MS analysis.
^e the predicted localisation of the peptide according to the presence of a N-terminal signal peptide (S), non-classical signal peptide (NC), transmembrane helices (TM), chloroplast (C), mitochondria (M) and unclassified (UC) location.
^f the peptide identification based on homology to proteins characterized in other species by Blast-p search on NCBI database.
^g the relative homology percentage of protein obtained by Blast-p.
Shared proteins identified in both AF sample and total leaf sample are highlighted in bold.

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ISSN: 1471-2229

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