Figure 5
High-resolution melting curves for amplicons of the co-dominant marker wri1 . Normalized and temperature- shifted difference plot for products amplified from genomic DNA of, Excalibur and 11 other cultivars known to carry Rlnn1 and/or known to carry Lr20/Sr15 (Group 1: Excalibur, Bindawarra, Bowie, Vectis, Wyalkatchem, Orion; Group 2: Aroona, Tatiara, Thew, Cascades; Group 3: BT- Schomburgk, Krichauff) and Kukri and 14 other cultivars known to lack Rlnn1 and/or known to lack Lr20/Sr15 (Group 4: Kukri, Sunbri, Calingiri, Sunlin, Sunvale, AGT Scythe, Annuello, Machete; Group 5: Sunstate, Chara, Babbler, Hartog, Buckley, Gladius, Yitpi).