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Table 3 Proteins significantly induced in leaf #16 after 35 d of sulphur restriction (Low S) identified by LC–MS/MS

From: Sulphur limitation provokes physiological and leaf proteome changes in oilseed rape that lead to perturbation of sulphur, carbon and oxidative metabolisms

Spot No.

Normalized spot volume (Mean±SE x1E+06)

Fold change

Exp. pI / Mr

Theo. pI / Mr

PM

Score

SC (%)

Protein name / Species / NCBI Accession number

 

Control

Low S

       

13

1.08

±0.18

12.47

±3.78

11.51

6.3 / 19

8.4 / 22.7

2

60

8

Water-soluble chlorophyll binding protein / Brassica oleracea var. acephala / gi|27530883

14

2.61

±0.40

13.75

±2.89

5.27

5.7 / 19

7.8 / 22.7

3

132

16

Water-soluble chlorophyll binding protein / Brassica oleracea var. acephala / gi|27530881

15

3.83

±1.88

11.77

±1.55

3.08

5.3 / 20

5.1 / 23.3

14

368

44

Trypsin inhibitor propeptide / Brassica oleracea / gi|841208

16

1.07

±0.09

3.11

±0.84

2.92

5.4 / 59

5.1 / 55.3

9

409

18

ATP synthase CF1 alpha subunit / Brassica napus / gi|262400756

17

2.97

±0.43

23.36

±1.06

7.86

6.6 / 29

6.5 / 28.8

7

175

15

BCA3 (BETA CARBONIC ANHYDRASE 4); carbonate dehydratase / zinc ion binding / Arabidopsis thaliana / gi|15220853

18

6.53

±0.32

9.69

±0.63

1.49

6.1 / 17

6.7 / 21.8

3

75

18

Cu-Zn Superoxide dismutase / Arabidopsis thaliana / gi|3273753

19

0.97

±0.15

2.22

±0.25

2.29

5.2 / 49

5.4 / 20.6

6

271

35

Putative myrosinase-binding protein 3 / Brassica rapa subsp. Pekinensis / gi|33285912

20

1.19

±0.13

2.73

±0.30

2.3

5.7 / 43

6.3 / 40.9

4

101

8

12-oxophytodienoate reductase / Arabidopsis thaliana / gi|2765083

21

2.82

±0.29

5.24

±0.70

1.86

5.4 / 51

7.6 / 48.7

2

77

5

Aminotransferase class I and II family protein / Arabidopsis thaliana / gi|15217440

22

8.10

±0.57

10.68

±0.44

1.32

5.2 / 57

5.0 / 54.7

21

954

50

Nucleotide-binding subunit of vacuolar ATPase / Arabidopsis thaliana / gi|166627

23

1.66

±0.25

4.81

±0.52

2.89

6.3 / 19

9.0 / 21.2

2

91

10

Unknown protein / Arabidopsis thaliana / gi|7658343

24

6.42

±0.36

14.89

±1.60

2.32

6.3 / 24

5.8 / 21.6

4

145

19

Unknown protein / Populus trichocarpa / gi|118485421

25

2.77

±0.22

8.29

±1.21

2.99

5.9 / 24

5.8 / 21.6

3

118

19

Unknown protein / Populus trichocarpa / gi|118485421

  1. Significant ANOVA was followed by a Mann–Whitney test (p≤0.05) carried out on the leaf–normalised spot volumes (n=3). Experimental and theoretical pI / M r , the number of LC–MS/MS matched peptides, the SCORE and the percentage of sequence coverage (SC) obtained are also indicated. The assigned best–matched protein is listed with the organism in which it was identified and its GenBank protein accession number.