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Table 3 Proteins significantly induced in leaf #16 after 35 d of sulphur restriction (Low S) identified by LC–MS/MS

From: Sulphur limitation provokes physiological and leaf proteome changes in oilseed rape that lead to perturbation of sulphur, carbon and oxidative metabolisms

Spot No. Normalized spot volume (Mean±SE x1E+06) Fold change Exp. pI / Mr Theo. pI / Mr PM Score SC (%) Protein name / Species / NCBI Accession number
  Control Low S        
13 1.08 ±0.18 12.47 ±3.78 11.51 6.3 / 19 8.4 / 22.7 2 60 8 Water-soluble chlorophyll binding protein / Brassica oleracea var. acephala / gi|27530883
14 2.61 ±0.40 13.75 ±2.89 5.27 5.7 / 19 7.8 / 22.7 3 132 16 Water-soluble chlorophyll binding protein / Brassica oleracea var. acephala / gi|27530881
15 3.83 ±1.88 11.77 ±1.55 3.08 5.3 / 20 5.1 / 23.3 14 368 44 Trypsin inhibitor propeptide / Brassica oleracea / gi|841208
16 1.07 ±0.09 3.11 ±0.84 2.92 5.4 / 59 5.1 / 55.3 9 409 18 ATP synthase CF1 alpha subunit / Brassica napus / gi|262400756
17 2.97 ±0.43 23.36 ±1.06 7.86 6.6 / 29 6.5 / 28.8 7 175 15 BCA3 (BETA CARBONIC ANHYDRASE 4); carbonate dehydratase / zinc ion binding / Arabidopsis thaliana / gi|15220853
18 6.53 ±0.32 9.69 ±0.63 1.49 6.1 / 17 6.7 / 21.8 3 75 18 Cu-Zn Superoxide dismutase / Arabidopsis thaliana / gi|3273753
19 0.97 ±0.15 2.22 ±0.25 2.29 5.2 / 49 5.4 / 20.6 6 271 35 Putative myrosinase-binding protein 3 / Brassica rapa subsp. Pekinensis / gi|33285912
20 1.19 ±0.13 2.73 ±0.30 2.3 5.7 / 43 6.3 / 40.9 4 101 8 12-oxophytodienoate reductase / Arabidopsis thaliana / gi|2765083
21 2.82 ±0.29 5.24 ±0.70 1.86 5.4 / 51 7.6 / 48.7 2 77 5 Aminotransferase class I and II family protein / Arabidopsis thaliana / gi|15217440
22 8.10 ±0.57 10.68 ±0.44 1.32 5.2 / 57 5.0 / 54.7 21 954 50 Nucleotide-binding subunit of vacuolar ATPase / Arabidopsis thaliana / gi|166627
23 1.66 ±0.25 4.81 ±0.52 2.89 6.3 / 19 9.0 / 21.2 2 91 10 Unknown protein / Arabidopsis thaliana / gi|7658343
24 6.42 ±0.36 14.89 ±1.60 2.32 6.3 / 24 5.8 / 21.6 4 145 19 Unknown protein / Populus trichocarpa / gi|118485421
25 2.77 ±0.22 8.29 ±1.21 2.99 5.9 / 24 5.8 / 21.6 3 118 19 Unknown protein / Populus trichocarpa / gi|118485421
  1. Significant ANOVA was followed by a Mann–Whitney test (p≤0.05) carried out on the leaf–normalised spot volumes (n=3). Experimental and theoretical pI / M r , the number of LC–MS/MS matched peptides, the SCORE and the percentage of sequence coverage (SC) obtained are also indicated. The assigned best–matched protein is listed with the organism in which it was identified and its GenBank protein accession number.