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Table 2 Proteins significantly repressed in leaf #16 after 35 d of sulphur restriction (Low S) identified by LC–MS/MS

From: Sulphur limitation provokes physiological and leaf proteome changes in oilseed rape that lead to perturbation of sulphur, carbon and oxidative metabolisms

Spot No. Normalized spot volume (Mean±SE x1E+06) Fold change Exp. pI / Mr Theo. pI / Mr PM Score SC (%) Protein name / Species / NCBI Accession number
  Control Low S        
1 11.16 ±1.07 7.85 ±0.29 −1.42 6.5 / 24 8.5 / 59.1 4 29 7 Glutathione S-transferase / Brassica oleracea / gi|171921127
2 12.49 ±0.39 9.55 ±0.35 −1.31 5.6 / 21 5.8 / 26.3 6 159 11 Photosystem I light-harvesting chlorophyll a / b-binding protein / Nicotiana tabacum / gi|493723
3 14.93 ±0.09 10.83 ±1.04 −1.38 6.7 / 33 8.7 / 41.1 5 204 17 FNR2 (Ferredoxin-NADP(+)-Oxidoreductase 2) / Arabidopsis thaliana / gi|145323954
4 9.39 ±0.63 5.21 ±0.37 −1.8 5.5 / 31 5.8 / 36.6 3 188 10 THI1; protein homodimerization / Arabidopsis thaliana / gi|15239735
5 1.70 ±0.06 1.31 ±0.08 −1.3 5.5 / 65 5.3 / 55.2 8 346 25 Mitochondrial chaperonin (HSP60) / Arabidopsis thaliana / gi|2924773
6 7.51 ±0.27 5.88 ±0.35 −1.28 6.4 / 35 8.5 / 42.3 13 516 39 Chloroplast malate dehydrogenase / Brassica rapa subsp. Pekinensis / gi|207667274
7 14.45 ±0.95 9.59 ±1.02 −1.51 5.8 / 19 6.8 / 21.8 3 112 17 Germin-like protein / Arabidopsis thaliana / gi|1755154
8 19.15 ±0.65 13.37 ±1.45 −1.43 6.2 / 19 6.8 / 21.8 3 94 17 Germin-like protein / Arabidopsis thaliana / gi|1755154
9 23.59 ±1.66 17.53 ±1.05 −1.35 6.2 / 20 6.8 / 21.8 3 102 17 Germin-like protein / Arabidopsis thaliana / gi|1755154
10 28.44 ±0.85 19.68 ±2.49 −1.45 6.8 / 30 6.2 / 27.5 14 619 55 Chain B, The Transient Complex Of Poplar Plastocyanin With Turnip Cytochrome F Determined With Paramagnetic Nmr / Brassica rapa / gi|67463833
11 2.90 ±0.20 2.14 ±0.17 −1.36 5.5 / 29 5.3 / 26.3 4 192 13 AT2G37660 / Arabidopsis thaliana / gi|227204455
12 9.24 ±1.30 5.96 ±0.37 −1.55 6.8 / 35 8.5 / 35.8 7 415 31 Mitochondrial malate dehydrogenase (NAD) / Arabidopsis thaliana / gi|18404382
  1. Significant ANOVA was followed by a Mann–Whitney test (p≤0.05) carried out on the leaf–normalised spot volumes (n=3). Experimental and theoretical pI / M r , the number of LC–MS/MS matched peptides (PM), the SCORE and the percentage of sequence coverage (SC) obtained are also indicated. The assigned best–matched protein is listed with the organism in which it was identified and its GenBank protein accession number.