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Table 2 Proteins significantly repressed in leaf #16 after 35 d of sulphur restriction (Low S) identified by LC–MS/MS

From: Sulphur limitation provokes physiological and leaf proteome changes in oilseed rape that lead to perturbation of sulphur, carbon and oxidative metabolisms

Spot No.

Normalized spot volume (Mean±SE x1E+06)

Fold change

Exp. pI / Mr

Theo. pI / Mr

PM

Score

SC (%)

Protein name / Species / NCBI Accession number

 

Control

Low S

       

1

11.16

±1.07

7.85

±0.29

−1.42

6.5 / 24

8.5 / 59.1

4

29

7

Glutathione S-transferase / Brassica oleracea / gi|171921127

2

12.49

±0.39

9.55

±0.35

−1.31

5.6 / 21

5.8 / 26.3

6

159

11

Photosystem I light-harvesting chlorophyll a / b-binding protein / Nicotiana tabacum / gi|493723

3

14.93

±0.09

10.83

±1.04

−1.38

6.7 / 33

8.7 / 41.1

5

204

17

FNR2 (Ferredoxin-NADP(+)-Oxidoreductase 2) / Arabidopsis thaliana / gi|145323954

4

9.39

±0.63

5.21

±0.37

−1.8

5.5 / 31

5.8 / 36.6

3

188

10

THI1; protein homodimerization / Arabidopsis thaliana / gi|15239735

5

1.70

±0.06

1.31

±0.08

−1.3

5.5 / 65

5.3 / 55.2

8

346

25

Mitochondrial chaperonin (HSP60) / Arabidopsis thaliana / gi|2924773

6

7.51

±0.27

5.88

±0.35

−1.28

6.4 / 35

8.5 / 42.3

13

516

39

Chloroplast malate dehydrogenase / Brassica rapa subsp. Pekinensis / gi|207667274

7

14.45

±0.95

9.59

±1.02

−1.51

5.8 / 19

6.8 / 21.8

3

112

17

Germin-like protein / Arabidopsis thaliana / gi|1755154

8

19.15

±0.65

13.37

±1.45

−1.43

6.2 / 19

6.8 / 21.8

3

94

17

Germin-like protein / Arabidopsis thaliana / gi|1755154

9

23.59

±1.66

17.53

±1.05

−1.35

6.2 / 20

6.8 / 21.8

3

102

17

Germin-like protein / Arabidopsis thaliana / gi|1755154

10

28.44

±0.85

19.68

±2.49

−1.45

6.8 / 30

6.2 / 27.5

14

619

55

Chain B, The Transient Complex Of Poplar Plastocyanin With Turnip Cytochrome F Determined With Paramagnetic Nmr / Brassica rapa / gi|67463833

11

2.90

±0.20

2.14

±0.17

−1.36

5.5 / 29

5.3 / 26.3

4

192

13

AT2G37660 / Arabidopsis thaliana / gi|227204455

12

9.24

±1.30

5.96

±0.37

−1.55

6.8 / 35

8.5 / 35.8

7

415

31

Mitochondrial malate dehydrogenase (NAD) / Arabidopsis thaliana / gi|18404382

  1. Significant ANOVA was followed by a Mann–Whitney test (p≤0.05) carried out on the leaf–normalised spot volumes (n=3). Experimental and theoretical pI / M r , the number of LC–MS/MS matched peptides (PM), the SCORE and the percentage of sequence coverage (SC) obtained are also indicated. The assigned best–matched protein is listed with the organism in which it was identified and its GenBank protein accession number.