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Figure 5 | BMC Plant Biology

Figure 5

From: Super-resolution imaging with Pontamine Fast Scarlet 4BS enables direct visualization of cellulose orientation and cell connection architecture in onion epidermis cells

Figure 5

Cellulose and callose around cell connections imaged with conventional confocal microscopy (A, B, E) and in combination with post-acquisition image deconvolution (C, D, F); a raw confocal image of PFS-stained cellulose in the cell wall between neighboring cells, b overlay with image of Anilin Blue-stained callose; After deconvolution the same images (C, D) show more clearly that callose and cellulose domains do not overlap; in maximum intensity projections a higher cellulose concentration can be clearly located around the callose depositions after deconvolution (F) compared to raw confocal images (E). Scale bars 10 μm.

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BMC Plant Biology

ISSN: 1471-2229

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