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Figure 4 | BMC Plant Biology

Figure 4

From: Super-resolution imaging with Pontamine Fast Scarlet 4BS enables direct visualization of cellulose orientation and cell connection architecture in onion epidermis cells

Figure 4

PFS-stained cellulose fibril orientation imaged with conventional confocal microscopy (A, C) and in combination with post-acquisition image deconvolution (B, D); Cellulose fibrils are at an angle close to 45° at a focal plane about 100 nm below the outer cell wall surface (A, B) compared to an angle around 90° at a focal plane about 450 nm below the outer cell wall surface (C, D); Blue lines indicate borders to neighboring cells. Scale bars 10 μm; 2 μm in inserts.

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BMC Plant Biology

ISSN: 1471-2229

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