Examples of data treatment by the CaSA software. The graphs derived from the confocal microscope records (see Additional file 7) are shown in A, B (values on the Y axis represent the ratio of YFP to CFP fluorescence); the corresponding output plots are presented in C, D, where the original data are shown in red, while the blue lines correspond to the SG filtered curves. The maximum of each peak detected by the software is marked by a green triangle; the estimated starting and ending points are marked by a blue circle and a green cross respectively. A, C show a representative record from a cell treated with the AM fungal exudate; a typical spiking profile is visible and the CaSA software efficiently identifies each peak. B, D present the corresponding data from a control cell treated with sterile distilled water where no Ca2+ peaks are recorded.