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Table 1 RS activities in leaves of wild-type (Col-0) and RS5 mutants subjected to various abiotic stresses

From: Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390)

  4°C WD NaCl MV 30°C
  NS S NS S NS S NS S NS S
Col-0 0.46 ± 0.046 1.86 ± 0.138 0.55 ± 0.014 1.17 ± 0.065 0.18 ± 0.005 0.34 ± 0.002 0.22 ± 0.004 0.33 ± 0.006 0.24 ± 0.004 0.33 ± 0.002
rs 5-1 0.15 ± 0.044 0.16 ± 0.028 0.16 ± 0.006 0.14 ± 0.002 n.d. n.d. n.d. n.d. n.d. n.d.
rs 5-2 0.11 ± 0.061 0.12 ± 0.025 0.17 ± 0.009 0.16 ± 0.002 n.d. n.d. n.d. n.d. n.d. n.d.
  1. Assays were conducted with desalted leaf crude extracts incubated with 100 mM Suc and 10 mM Gol at pH 7.5 for 1 h. NS, non-stressed; S, stressed (as per Materials and Methods); 4°C, cold-stressed; WD, water deficit-stressed; NaCl, salt-stressed; MV, methyl viologen-stressed; 30°C, heat-stressed. Values in bold represent increased RS activities in stressed as compared to non-stressed leaves. Values are the means ± SE of three independent replicates and represent RS activities expressed in nkat g-1 DW.