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Table 3 Candidate genes significantly regulated within the RNA-Seq experiment (FDR < 0.05) and validated by RT-PCR

From: Ups and downs of a transcriptional landscape shape iron deficiency associated chlorosis of the maize inbreds B73 and Mo17

Gene

Position

Function1

Accession2

Comp. 1

Comp. 2

Comp. 3

Comp. 4

(log2FC)

(log2FC)

(log2FC)

(log2FC)3

GRMZM2G350312

1:65657063-65660290

bHLH transcription factor

AT3G47640.1(PYE)/LOC_Os03g26210.2/B8A2P8

sig. (1.81)

  

sig. (3.07)

GRMZM2G106980

1:203647045-203653762

Putative BURP-domain protein, uncharacterized protein

AT5G25610.1(ATRD22,RD22)/LOC_Os06g19800.1/B8A121

sig. (-3.80)

sig. (-4.25)

  

GRMZM2G059465

1:209119390-209120903

O-methyl transferase, uncharacterized protein

AT4G35160.1/LOC_Os06g16960.1/B8A095

   

sig. (-2.49)

GRMZM2G055802

1:293997055-290998873

NA

LOC_Os03g60840.1

  

sig. (2.53)

 

GRMZM2G132678

3:20795800-20806607

Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase

AT5G48930.1(HCT)/LOC_Os08g43040.1

sig. (5.35)

sig. (4.50)

  

GRMZM2G057413

3:148031451-148062994

bHLH transcription factor

AT3G56970.1(BHLH038,ORG2)/LOC_Os01g72370.1

   

sig. (3.77)

GRMZM2G400602

3:174361241-174363882

Putative oligopeptide transporter, uncharacterized protein

AT1G22540.1/LOC_Os01g65100.1/C0HEP6

   

sig. (3.29)

GRMZM5G896496

3:211872426-211879044

Putative chaperone ClpC1, uncharacterized protein

C0HH28

sig. (-5.10)

sig. (-5.00)

  

GRMZM2G574782

4:18676198-18682894

Probable bifunctional methylribose-1-phosphate dehydratase/enolase phosphatase E1

AT5G53850.2/LOC_Os11g29370.1/B4G0F3

sig. (1.74)

  

sig. (1.92)

GRMZM2G096958

4:219581607-219585123

Nicotinamine aminotransferase1, uncharacterized protein

AT5G36160.1/LOC_Os02g20360.1/B4FEZ7

sig. (1.71)

  

sig. (1.98)

GRMZM2G010280

4:240232549-240234575

High affinity nitrate transporter

AT5G60770.1(ATNRT2.4,NRT2.4/LOC_Os02g02170.1)/Q53CL7

   

sig. (-3.12)

GRMZM2G010251

4:240243499-240245403

High affinnity nitrate transporter, uncharacterized protein

AT1G08090.1(ACH1,ATNRT2.1)/LOC_Os02g02170.1/B4FSV9

   

sig. (-2.58)

GRMZM2G089836

567508591-67511890

Glycosyl hydrolase, Invertase

AT1G62660.1/LOC_Os02g01590.1/B6T0A9

   

sig. (-2.61)

GRMZM2G041980

5:148304652-148307020

Putative aquaporin protein, NIP1-1

AT4G18910.1(ATNLM2,NIP1)/LOC_Os05g11560.1/Q9ATN4

sig. (3.46)

 

sig. (-2.41)

 

GRMZM2G465226

7:3483731-3484794

Pathogenesis related protein 1 (PR-1), uncharacterized protein

AT5G57625.1: CAP/LOC_Os07g03710.1/B4FVP5

 

sig. (3.71)

sig. (1.83)

 

GRMZM2G099049

7:120764735-120766193

Auxin/Dormancy related protein

AT1G56220.2/LOC_Os09g26620.1

   

sig. (-2.60)

GRMZM2G336694

8:10989824-10990619

NA

NA

sig. (3.67)

sig. (3.38)

  

GRMZM2G153977

8:70995815-70998984

Eukaryotic aspartyl protease

AT1G62290.1/LOC_Os05g49200.1

 

sig. (-4.24)

  

GRMZM2G096029

8:104279085-104281118

Obtusifoliol 14α demethylase

AT1G11680.1(CYP51)/LOC_Os05g34325.1

   

sig. (-2.91)

GRMZM2G135536

10:893975-895713

Putative cytochrome P450

AT1G64940.1(CYP89A6)/LOC_Os10g05020.1

sig. (3.51)

sig. (4.24)

  

GRMZM2G133721

10:111856375-111861824

Putative enolase

NA

sig. (7.65)

sig. (6.36)

  
  1. 1Annotations from http://www.maizegdb.org.
  2. 2Accession number for the homologous sequences from Arabidopsis thaliana, Oryza sativa (database information from http://www.maizegdb.org), and UniProtKB/TrEMBL.
  3. 3log2FC for the corresponding comparison in which the gene was identified as being differentially expressed (significant at FDR < 0.05) positive log2FCs reflect higher expression values in Condition2 whilst negative log2FC refer to higher expression in Condition 1 of specific comparisons (cf Table 1).