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Table 1 Validated miRNA targets

From: Genome-wide characterization of microRNA in foxtail millet (Setaria italica)

Target ID miRNA ID Target start-end 5′-target sequence-3′ complementary pattern 3′-miRNA sequence-5′ InterPro description of targets
   1767(9/10) B3 DNA binding domain Aux/IAA-ARF-dimerisation DNA-binding pseudobarrel domain AUX/IAA protein
Si005991m 1756-1776 5'-AGGCAUACAGGGAGCCAGGCA-3'
sit-miR160   3'-ACCGUAUGUCCCUCGGUCCGU-5'
   1364(9/10) AUX/IAA protein B3 DNA binding domain Auxin response factor Aux/IAA-ARF-dimerisation DNA-binding pseudobarrel domain
Si016509m 1353-1373 5'-AGCCAUACAGGGAGCCAGGCA-3'
sit-miR160   3'-ACCGUAUGUCCCUCGGUCCGU-5'
   1328(6/10) AUX/IAA protein B3 DNA binding domain Auxin response factor Aux/IAA-ARF-dimerisation DNA-binding pseudobarrel domain
Si034525m 1317-1337 5'-AGGCAUACAGGGAGCCAGGCA-3'
sit-miR160   3'-ACCGUAUGUCCCUCGGUCCGU-5'
   1085(7/10) Transcription factor GRAS
Si016508m 1074-1094 5'-GAUAUUGGCGCGGCUCAAUCA-3'
sit-miR171a   3'-CUAUAACCGCGCCGAGUUAGU-5'
sit-miR171b   3'-CUAUAACCGUGCCGAGUUAGU-5'
   1082(2/10) Transcription factor GRAS
Si016508m 1071-1091 5'-AGAGAUAUUGGCGCGGCUCAA-3'
nov-sit-miR15   3'-UCUCUAUAACCGCGCCGAGUU-5'
nov-sit-miR14   3'-CCUCUAUAACUGCGCCGAGUU-5'
nov-sit-miR49   3'-UCACUAUAACCGAGCCGAGU-5'
  1. Mapping of mRNA cleavage sites by RNA Ligase-Mediated 5′ RACE. The complementary pattern of miRNA sequences and partial sequences of the target mRNAs are shown in the table. All predicted mismatch base parings are shown in bold letters. The positions inferred as 5′ ends of miRNA-guided cleavage products, as revealed by 5′ RACE, and the numbers of sequenced 5′ RACE clones corresponding to each site are indicated by vertical arrowheads.