GO functional enrichment analysis for the predicted target genes of the foxtail millet miRNAs and validations of the smRNA-seq results. (A) GO functional enrichment analysis for the target genes was compared with the classification for all the foxtail millet genes retrieved from the Phytozome database. (B) Stem-loop RT-PCR validation of the smRNA-seq results from foxtail millet (Setaria italica). The RT-qPCR expression profiles (blue bars) match the smRNA-seq data (red lines) closely for ten miRNAs we tested. The correlation value (cor) was calculated using Pearson’s product–moment correlation.