Response of seed germination and cotyledon growth of transgenic and lcr plants to ABA. A/ B/, E/ F, I/ J: Growth of wild-type, 35S::MIR394a (A/B), lcr (E/F) and 35S::m5LCR (I/J) plants on MS medium with 0.5 and 1 μM ABA. Seeds were germinated and grown for 10 d (35S::MIR394a and lcr plants), and 7 d (35S::m5LCR plants). C/ D, G/ H, K/ L: ABA-dependent seed germination (C/G/K) and cotyledon greening (D/H/L). Seeds of wild-type, 35S::MIR394a (C/D), lcr (G/H) and 35S::m5LCR (K/L) plants were germinated plates containing 0.25-2 μM ABA. Seed germination were determined after 4 d and cotyledon greening were determined after 7 d. For 35S:MIR394b plants, similar results were found (data not shown). Vertical bars represent standard deviation of the mean (n =3, treatments with 60–120 seedlings). Asterisks indicate the significant difference between the transgenic lines/mutants and wild-type (WT) (P<0.05).