Responses of transgenic plants to drought. A/B/C: Three week-old wild-type, 35S::MIR394a (A), lcr mutant (B) and 35S::m5LCR (C) plants were dehydrated for 14 (A and B) or 12 (C) d, followed by rewatering for 3 d. Dehydration tolerance was assayed as the ability of plants to resume growth when returned to normal conditions. D: Survival of wild-type, 35S::MIR394a and lcr mutant plants by rewatering for 3 d after dehydration treatment for 14 d. E: Survival of wild-type and 35S::m5LCR plants by rewatering for 3 d after dehydration treatment for 12 d. F: Measurement of water loss rates. Detached rosette leaves of the indicated plants were placed on open-lid Petri-dishes and weighed at the indicated times after excision. Water loss was calculated as the percentage of initial fresh weight. For 35S:MIR394b plants, similar results were found (data not shown). Vertical bars represent standard deviation of the mean (n = 3, treatments with 60–100 seedlings). Asterisks indicate the significant difference between the transgenic lines/mutants and wild-type (WT) (P<0.05).