Figure 3

Characterization of transgenic plants and lcr mutant lines. (A) Assessment of LCR protein in plants grown on MS medium for 14 d. (B) Schematic diagram of LCR structure and T-DNA diagnostic PCR and RT-PCR. Closed boxes represent exons, opened boxes represent the 5′ and 3 UTR, solid line between closed boxes represents introns and dash line represents promoter region. Gene-specific (forward and reverse) and T-DNA–specific (LB1.3) primers used in the genotyping and RT-PCR are shown with arrows. (C) and (D) Diagnostic PCR of T-DNA inserted in the two different regions of LCR. DNA from insertion lines of lcr-1 (SALK_016763c) and lcr-2 (SALK_136833c) were used. (E) RT-PCR analysis of the LCR transcripts in wild-type and T-DNA insertion mutant seedlings. (F) Detection of the LCR protein in wild-type and lcr seedlings.