Figure 10

Real-time qRT-PCR analysis of ABA-responsive genes in 35S::MIR394a and 35S::m5LCR plants. Two week-old wild-type and transgenic plants were treated with 100 μM ABA for 6 h. Total RNA was isolated from the plants and analyzed by qRT-PCR using the gene-specific primers listed in Additional file 1: Table S1. The graphs indicate the induction fold of the genes with 100 μM ABA as compared with the control (0 μM ABA). For 35S:MIR394b plants, similar results were found (data not shown). A: The first group genes, ABI3, ABI4, ABI5, ABF3, and ABF4; and B: The second group genes, RD29A, KIN1, RD22 and P5CS. Vertical bars represent standard deviation of the mean (n =3, treatments with 60 seedlings). Asterisks indicate the significant difference between the transgenic lines/mutants and wild-type (WT) (P<0.05).