Fragment number
|
Primer name
|
Sequence (5′-3′)
|
Position of fragment within theVRN-A1gene (bp from start of Genbank accession AY747600)
|
Size of amplified fragment (bp)
|
Number of restriction site within amplicon for:
|
---|
| | | | |
MspI
|
PspGI
|
BstUI
|
---|
0.0 k
|
VRN_1_A_pr_F*
|
GAAAGGAAAAATTCTGCTCG
|
44–527
|
483
|
8
|
1 (n.a.)
|
3
|
VRN_1_ex1_R*
|
TGCACCTTCCCSCGCCCCAT
|
1.6 k
|
1.6 k-F
|
GCCTCCACGGTTTGAAAGTA
|
1695–2358
|
663
|
1
|
0
|
2
|
1.6 k-R
|
ATCTCAAGATTTTAGTTCCGATCCT
|
3.0 k
|
3.0 k-F
|
TGCTGCAGTGATATTTTGTTAGC
|
3030–3710
|
680
|
0
|
1
|
0
|
3.0 k-R
|
TGATGGGTCATAAGGTTTTGC
|
4.0 k
|
4.0 k-F
|
CTTCCTTGGTGGGCTGTG
|
4003–4607
|
604
|
1
|
0
|
1
|
4.0 k-R
|
TGGCTCTCCACCACAATACC
|
5.0 k
|
5.0 k-F
|
GGCTAAGATCGTGAGGAAGG
|
5014–5648
|
634
|
0
|
1
|
0
|
5.0 k-R
|
TGACTAGCACCACATCAATCG
|
6.8 k (b)
|
6.8 k (b)-F
|
GCGGCATCATCTTCTTGC
|
6834–7148
|
314
|
1
|
0
|
2
|
6.8 k (b)-R
|
GGCTACTCCTCCACCTTATGC
|
9.8 k
|
9.8 k-F
|
TCTCCAGTCCTTCGGATTGT
|
9871–10489
|
618
|
1
|
1
|
0
|
9.8 k-R
|
GGCTTTTGGGTTTCATCTCC
|
11.0 k
|
11.0 k-F
|
AATGATTTGATACAGCAGCACAATA
|
11028–11710
|
682
|
1
|
1
|
0
|
|
11.0 k-R
|
ACCAATTCAAAAGATGGTTACTTGA
| | | | | |
- *PCR primers from [41]. Abbreviations: n.a.: not analyzed.