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Table 2 Recognition sites and methylation sensitivities of the restriction enzymes used

From: Vernalization treatment induces site-specific DNA hypermethylation at the VERNALIZATION-A1 (VRN-A1) locus in hexaploid winter wheat

Enzyme Recognition site Sensitivity to methylation
Bfa I none
BstU I CG
Hpa II CG
Msp I CHG
PspG I CHG/CHH
  1. Arrowheads indicate enzymes cutting sites and bold Cs show methylation sensitive sites.