Figure 3

DNA methylation levels for 4^thleaf, flag leaf and progeny 4^thleaf. A. Fragment 6.8 k (a). B. Fragment 7.6 k. Results of both G1 and G2 were pooled. Red, blue and green colors represent CG, CHG and CHH contexts, respectively. Faint and strong colors represent non-vernalized and vernalized plants respectively. From left to right: DNA methylation from on 4^th leaf for two independent sets of experiments (exp 1 and exp 2), from the flag leaf of exp 2 and for the 4^th leaf of the progeny (exp 2). In experiment 1, results from three replicates of G1 and three replicates of G2 were pooled. In experiment 2, results from two replicates of G1 and two replicates of G2 were pooled. Stars highlight statistical significance of methylation variation induced by vernalization treatment, with ***p < 0.001.