Heat-responsive nat-siRNAs derived from Bra018217/Bra018216 cis-NAT pair and expression of their homologs in Arabidopsis. (A) Assembled transcript models of Bra018217 in four RNA-seq datasets. Blue rectangles indicate the original coding sequence model without untranslated regions; red rectangles indicate Brassica rapa ssp. pekinensis cv. Bre seedling; orange rectangles indicate in B. rapa ssp. chinensis cv. Wut seedling; green rectangles indicate cv. Bre inflorescence apex; pink rectangles indicate cv. Wut inflorescence apex. (B) Nat-siRNAs were derived from the strands of Bra018216 (top of gene pairs) or of Bra018217 (bottom). The red lines indicate heat-induced nat-siRNAs in high-temperature libraries, and the black one indicates nat-siRNAs in normal-tmperature libraries. Top right corner is the northern bolt result of nat-siRNAs that derived from the strand of Bra018216 under normal treatment (NT) and heat treatment (HT). (C) Expression of At3G46230, a homolog of Bra018216, and At3G46220, a homolog of Bra018217, under abiotic stress (AtGenExpress Visualization Tool).