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Table 1 The level of total amino acids in dry wild type (WT) seeds, in seeds expressing F-AtCGS (LF) and in seeds expressing T-AtCGS (LT), after protein hydrolysis

From: Tobacco seeds expressing feedback-insensitive cystathionine gamma-synthase exhibit elevated content of methionine and altered primary metabolic profile

  WT LF 29 LF 17 LT 19 LT 30
Alanine 19232 ± 1957 20778 ± 2289 21704 ± 1098 22891 ± 1713 21993 ± 1665
Valine 41269 ± 6044 41332 ± 6002 41269 ± 3322 43454 ± 1418 45885 ± 3045
Serine 18725 ± 1181 B 18090 ± 3097 B 18725 ± 2660 B 28859 ± 130 A 22908 ± 127 A
Leucine 45675 ± 7705 45499 ± 5528 45531 ± 6186 53449 ± 985 55274 ± 1937
Threonine 23410 ± 4732 23187 ± 4212 23410 ± 3357 34249 ± 1026 31440 ± 2247
Isoleucine 30985 ± 4603 31254 ± 1233 31981 ± 1966 32913 ± 599 39781 ± 1098
Proline 11234 ± 1891 B 10954 ± 985 B 11164 ± 1550 B 13196 ± 1439 A 13784 ± 726 A
Glycine 108546 ± 4480 108756 ± 3567 109546 ± 5814 109494 ± 6499 112348 ± 4972
Methionine 4374 ± 315 B 3962 ± 997 B 4199 ± 313 B 6992 ± 459 A 6376 ± 573 A
Aspartate 39625 ± 11058 B 41500 ± 9876 B 40625 ± 7303 B 61360 ± 2182 A 48541 ± 3687 A
Phenylalanine 21500 ± 4772 23058 ± 5562 22003 ± 3568 27960 ± 1025 28166 ± 2235
Glutamate 3058 ± 785 B 2984 ± 689 B 3644 ± 330 B 9217 ± 242 A 5896 ± 564 A
Histidine 2725 ± 666 2769 ± 1072 2863 ± 605 3415 ± 358 3722 ± 231
Lysine 20263 ± 4330 22645 ± 5562 22094 ± 4570 25838 ± 1138 24113 ± 2645
Tyrosine 19841 ± 3273 20476 ± 5797 21195 ± 3165 24230 ± 1115 27420 ± 971
Total 411462 ± 21890 B 417244 ± 18664 B 419953 ± 11284 B 497517 ± 19064 A 487647 ± 13687 A
  1. The levels of amino acids were measured using GC-MS and displayed as nmol/gr dry seeds. Values are representative of two independently grown sets of plants are the mean ± standard deviation of five biological repetitions of 50 mg seeds isolated from three plants per line. Statistically significant changes (p < 0.05, using two-way ANOVA) are identified by letters.