The level of cysteine (Cys) and glutathione (GSH) in seeds of wild type (WT) and homozygous transgenic tobacco seeds expressing the two forms of Arabidopsis CGS: the full-length AtCGS (LF), and the truncated form of AtCGS (LT). Two transgenic events for each line were analyzed. The left panel in each box described the level of Cys or GSH in WT and transgenic seeds, while the right panel described the results obtained from the feeding experiments performed on a receptacle of developing capsules of WT fed with 5 mM Met or DDW as a control. The quantities of these two metabolites were analyzed using HPLC. The data presented represent the mean ± SD of four samples taken from three different plants for each line. Statistically significant changes (P <0.05, using two-way ANOVA) are identified by different letters.