Figure 3

Soluble amino acid contents in seeds of wild type (WT) and homozygous transgenic tobacco seeds expressing the two forms of Arabidopsis CGS: the full-length AtCGS (LF) and the truncated form of AtCGS (LT) whose levels were significantly altered from those found in WT. Two transgenic events for each line were analyzed (LF17; LF29 and LT19; LT30). The left panel in each box of amino acid described the level of this amino acid in WT and transgenic seeds, while the right panel in each box described the results obtained from the feeding experiments performed on a receptacle of developing capsules of WT fed with 5 mM Met or DDW as a control. The quantities of amino acids were analyzed using GC-MS, and their levels were normalized to norleucine and calculated as nmol per g fresh weight of seeds. The data presented represent the mean ± SD of four samples taken from three different plants for each line. Statistically significant changes (P <0.05, using two-way ANOVA) are identified by different letters only when the values were statistically different.