Epigenetic rather than genetic factors may explain phenotypic divergence between coastal populations of diploid and tetraploid Limonium spp. (Plumbaginaceae) in Portugal

Table 4 MSAP fragment number analysis

Ploidy level	Species	E1/H1		E1/H3		Total
Ploidy level	Species	HpaII	MspI	HpaII	MspI	HpaII	MspI
Diploid	*L. lanceolatum*	52	52	47	38	99	90
	*L. nydeggeri*	50	48	43	33	93	81
	*L. ovalifolium*	48	43	42	28	90	71
Tetraploid	*L. dodartii*	37	77	24	56	61	133
	*L multiflorum*	33	59	30	35	63	94
	*L. vulgare*	26	46	20	39	46	85

Average number of MSAP fragments per diploid (L. lanceolatum, L. nydeggeri, L. ovalifolium) and tetraploid (L. dodartii, L. multiflorum and L. vulgare) species obtained using isoschizomers enzymes HpaII (methylation sensitive) and MspI (methylation insensitive) and primer combinations E1/H1 and E1/H3.

ISSN: 1471-2229