Figure 5
From: TcNPR3 from Theobroma cacao functions as a repressor of the pathogen defense response
Pathogen bioassays of cacao leaves transiently transformed with a NPR3 microRNA. A. Relative expression of NPR3 in cacao leaves by RT-Q-PCR. RNA samples were collected two days after transformation by Agrobacterium vacuum infiltration. Genotypes are indicated on x-axis as: Ctrl: control vector. MiRNA-NPR3: micro RNA of cacao NPR3 derived from mir319a in Arabidopsis. B. Representative images of transformed leaves three days after inoculation with agar plugs containing Phytophthora capsici mycelium on the right and agar plugs with water on the left. Size bars are indicated in white (10 mm). C. Average lesion sizes (mean ± SE of 12 lesion spots from four leaf discs). D. Relative virulence of P. capsici was measured by DNA RT-Q-PCR to calculate the ratio of pathogen actin to cacao actin three days after inoculation. Bars represent mean ± SE of four biological replicates each with three technical replicates. Asterisks show statistically significant difference to Ctrl determined by ANOVA.