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Table 5 Sequences of the species-specific primers that amplify a part of the ITS1 region

From: Pythium species from rice roots differ in virulence, host colonization and nutritional profile

Pythiumisolate Forward ITS primer (5′-3′)a Reverse ITS primer (5′-3′)a Size amplicon GenBank Accession no.b
P. arrhenomanes PT 60 ATTCTGTACGCGTGGTCTTCCG (3 μM) ACCTCACATCTGCCATCTCTCTCC (1 μM) 311 bp HQ877857
P. graminicola PB912 132 ATGGCTGAACGAAGGTGGGCTG (1 μM) TCCCGAAAGTGCAATGTGCGTTC (3 μM) 240 bp HQ877865
P. inflatum PT 52 AGGTGGGCGCATGTATGTGTGTC (500 nM) ACGTATCGCAGTTCGCAGCG (3 μM) 165 bp HQ877856
  1. aThe concentrations for each primer stock are presented between brackets.
  2. bThe ITS-regions of P. arrhenomanes PB912 75 (HQ877857) and P. graminicola PB912 116 (HQ877865) are respectively identical to those of P. arrhenomanes PT 60 and P. graminicola PB912 132 [3].