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Table 2 In planta quantification of Pythium DNA (pg/ng total DNA) using qPCR

From: Pythium species from rice roots differ in virulence, host colonization and nutritional profile

 

P. arrhenomanes*

P. graminicola*

P. inflatum*

 

Exp 1

Exp 2

Exp 1

Exp 2

Exp 1

Exp 2

A

1 dpi

84.24 (0.40)

179.26 (17.85)

57.76 (4.70)

179.64 (8.94)

13.88 (0.64)

2.89 (0.27)

2 dpi

492.00 (28.28)

291.42 (19.37)

47.28 (0.85)

142.00 (30.60)

264.10 (03.65)

113.44 (2.09)

3 dpi

590.00 (36.77)

405.32 (82.99)

58.40 (1.58)

51.14 (10.78)

157.70 (25.37)

196.16 (6.79)

B

20 hpi

1.246 (0.141)

2.162 (2.437)

0.330 (0.027)

0.031 (0.001)

0.010 (0.006)

0.003 (0.001)

28 hpi

8.434 (0.240)

8.782 (0.167)

8.958 (0.325)

1.128 (0.082)

0.966 (0.040)

0.108 (0.006)

73 hpi

47.680 (4.299)

4.564 (0.238)

55.320 (6.505)

0.332 (0.040)

0.857 (0.157)

0.019 (0.001)

  1. *In each experiment (Exp 1 and 2) 12 rice seedlings were pooled per treatment at various times. The presented data are means of two technical replicates (n = 2). Values between brackets represent standard deviations.
  2. A, Concentration of Pythium DNA in non-surface sterilized rice seedlings roots. Seeds were germinated on Gamborg B5 medium and inoculated at three days post imbibition. Root samples were collected in two different experiments at various times upon inoculation. B, Concentration of Pythium DNA in surface sterilized rice seedling roots. Seeds were germinated on plant agar-medium and inoculated at three days post imbibition. Rice roots were collected in two different experiments at various times upon inoculation and were surface-sterilized prior to processing.
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BMC Plant Biology

ISSN: 1471-2229

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