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Figure 7 | BMC Plant Biology

Figure 7

From: Proanthocyanidin synthesis in Theobroma cacao: genes encoding anthocyanidin synthase, anthocyanidin reductase, and leucoanthocyanidin reductase

Figure 7

Complementation of the Arabidopsis PA-deficient ldox mutant by constitutively expressing TcLAR . A, Catechin and epicatechin standards analyzed by HPLC. B, HPLC analysis of Col-0 young siliques. C, HPLC analysis of ldox 35S:TcLAR Line14 young siliques. D, HPLC analysis of ldox mutant young siliques. E. TcLAR and AtUbiquitin transcripts in total RNA from leaves of ldox 35S:TcLAR transgenic plants, (line 9, line 14 and line 36), Col-O and ldox muant plants by RT-PCR. PCR products from the TcLAR-PGEM plasmid were loaded on the last lane as a positive control for the TcLAR primer set and as a negative control for the AtUbiquitin primer set. F, Catechin and epicatechin levels in young siliques of plants that were the source of the total RNA used in (E). Catechin and epicatechin levels were determined by extraction and HPLC analysis. The data are presented as means ± SE, n = 3. *P < 0.05 versus ldox; **P < 0.01 versus ldox. FW, fresh weight; EU, emission units.

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BMC Plant Biology

ISSN: 1471-2229

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