Figure 6

Characterization of transgenic tobacco flowers constitutively expressing TcANS or TcLAR . A, Pigmentation of flower petals from wild type (WT) and two independent lines of TcANS transgenic (ANS12E3, ANS12H2) tobacco plants. B, Analysis of TcANS and NtrRNA transcripts in total RNA from leaves of plants shown in (A) by RT-PCR. PCR products from TcANS-pGEM plasmid were loaded on the last lane acting as a positive control for TcANS primer set and a negative control for the NtrRNA primer set. C, Anthocyanin levels in flower petals of plants shown in (A). D, Total soluble PA levels in flower petals of plants shown in (A). E, Flowers from wild type (WT) and two independent lines of TcLAR transgenic (LAR7C1, LAR9A2) tobacco plants. F, Analysis of TcLAR and NtrRNA transcripts in total RNA from young leaves of plants shown in (E) by RT-PCR. PCR products from the TcLAR-PGEM plasmid alone were loaded on the last lanes to act as a positive control for the TcLAR primer set and a negative control for the NtrRNA primer set. G, Anthocyanin levels in flower petals of WT and transgenic plants. H, Total soluble PAs levels in flower petals of WT and transgenic plants. I. Flavan-3-ol accumulation and composition in flowers of wild type (Samsun, ss) tobacco plants and two independent lines of 35S:TcLAR transgenic (LAR7C1, LAR9A2) tobacco plants. Anthocyanin levels were determined by extraction and UV absorption with cyanidin 3-glucoside as standards. Total soluble PA levels are determined by extraction and DMACA reaction with procyanidin B2 as standards. Flavan-3-ol levels were determined by extraction, HPLC separation, and quantification. All data are presented as mean values ± ≥SE,7.*Pn < 0.05 versus WT; **P < 0.01 versus WT.