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Figure 5 | BMC Plant Biology

Figure 5

From: Proanthocyanidin synthesis in Theobroma cacao: genes encoding anthocyanidin synthase, anthocyanidin reductase, and leucoanthocyanidin reductase

Figure 5

Complementation of the PA deficient Arabidopsis ban mutant phenotype by constitutive expression of TcANR . A, 7-day-old seedlings and DMACA-stained seeds from Col-0 the ban mutant (SALK_040250) and four independent transgenic lines (ban35S:TcANR). The bar represents 1 mm. B, Analysis of TcANR and AtUbi transcripts in total RNA from leaves of plants shown in (A) by semi-quantitative RT-PCR. PCR products from TcANR-pGEM plasmid were loaded on the last lane acting as a positive control for the TcANR primer set and a negative control for the AtUbi primer set. C, PA levels in mature seeds of plants shown in (A). PA levels were determined by extraction and DMACA reaction with procyanidin B2 as standards. All data are presented as mean values ± SE, n = 3. *P < 0.05 versus ban; **P < 0.01 versus ban.

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