Figure 3
Phylogenetic analysis of the LAR, ANS and ANR proteins as well as related IFR and DFR proteins of the RED superfamily. The protein sequences were aligned as described in Material and Methods. The scale bar represents 0.2 substitutions per site. Numbers indicate bootstrap values. The tree includes LAR, ANR and ANS proteins from cacao, which are shown in bold font, as well as from other species whose enzymatic activities have been shown in previous publications. Also included in the tree are 8 IFR like proteins from Arabidopsis (labeled with their locus numbers). All the other proteins are labeled by the species they come from followed by their catalytic activities. The species represented and their GenBank accession numbers in the LAR group are Medicago (MtLAR, CAI56327), Vitis vinifera (VvLAR, CAI26309), Desmodium uncinatum (DuLAR, CAD79341), Malus domestica (MdLAR1, Q5D7Y1; MdLAR2, Q5D7Y2) and Lotus corniculatus (LcLAR1-1, ABC71324; LcLAR1-2, ABC71325); in the DFR group are Zea mays (ZmA1, CAA28734), Arabidopsis thaliana (AtDFR, NP_199094) and Medicago truncatula (MtDFR, AAR27014); in the ANR group are Arabidopsis thaliana (BAN, NP_176365), Medicago truncatula (MtANR, AAN77735), Vitis vinifera (VvANR, CAD91911), Camellia sinensis (CsANR, AAT68773) and Lotus corniculatus (LcANR, ABC71337); and in the ANS group are Perilla frutescens (PfANS, O04274), Arabidopsis thaliana (AtANS, Q96323), Zea mays (ZmA2, CAA39022) and Medicago truncatula (MtANS, ABU40983). The sequence alignment used to generate this figure can be found in “Additional file 1: Figure S1”.