Figure 3
Sequence comparison of LgDes with experimentally validated homologs. Alignment was made using CLUSTALW2 [28] and BOXSHADE 3.21. (http://www.ch.embnet.org/software/BOX_form.html). Residues identical for six or more sequences in a given position are in white text on a black background, and for six or more similar residues are white with grey background. Sequences: LgDes (KF638283, this study), Δ6 desaturase sequences: Borago officinalis (BoD6, AAC49700) [13]; Echinum gentianoides (EgD6, AAL23580) [14], Primula farinosa (PfD6, AAP23034) [29], Ribes nigrum (RnD6, ADA60230) [30], Primula vialii (PvD6, AAP23036) [29]. Δ8 sequences: Arabidopsis thaliana (AtD8a, NP_191717; AtD8b, NP_182144) [26], Nicotiana tabacum (NtD8, ABO31111) [17], Helianthus annuus (HaD8, CAA60621) [31], Primula farinosa (PfD8, AAP23033, PvD8, AAP23035) [29]. Three histidine boxes common to membrane bound desaturases are underlined in red. The consensus cytochrome b5 sequence is underlined in blue and the amino acid position corresponding to L210 of LgDes, identified by CPDL, is marked with a vertical red arrow.