Figure 7
Chromocenter organization is altered in crwn1 crwn2 and crwn4 mutants. (A and B) Fluorescence in situ hybridization of representative small and large nuclei from wild type, crwn1 crwn2 and crwn4 cells prepared from leaves of adult plants after bolting. Blue: DAPI, pink: 180-bp centromere repeats, green: 5S RNA genes, bar, 5 μm. (C) The distribution of nuclei based on the number of centromere repeat clusters in different genotypes. Each circle represents an individual nucleus in the FISH experiment. The color indicates the level of decondensation of the centromere signal. (D) The distribution of nuclei based on the number of 5S RNA repeat clusters in different genotypes. Each circle represents an individual nucleus. The color indicates the level of dispersion of the 5S repeat signal.