Table 3 Details of the genic (EST) SSR markers developed for mulberry
Sl no | Primer name | Primer sequence | GenBank-ID | Amplicon size | Repeat motif | Ta (0°C) | Repeat type |
|---|---|---|---|---|---|---|---|
1 | MESTSSR10F | CATTGCACATTGCAGGTAGC | GT629469.1 | 237 | GTT | 52.8 | Perfect |
| Â | MESTSSR10R | CGGCCATCCAAAATGTTGTTC | Â | Â | Â | Â | Â |
2 | MESTSSR13F | TCTATCTCAACCGGAAGTCC | GT628644.1 | 230 | (CAAAAG)G(AAAATA) | 54.8 | interrupted |
| Â | MESTSSR13R | CCAATTTGCTCGTCTTATGC | Â | Â | Â | Â | Â |
3 | MESTSSR14F | CGGCCACAGGTACTTTC | GT628768.1 | 202 | TTGATT | 50.4 | Perfect |
| Â | MESTSSR14R | GGCAGCGATTTAGGATTGG | Â | Â | Â | Â | Â |
4 | MESTSSR20F | CGCAAGTGTCTCAACTG | GT629110.1 | 200 | TGA | 49.1 | Perfect |
| Â | MESTSSR20R | GGAACGGATGGAGTAAG | Â | Â | Â | Â | Â |
5 | MESTSSR23F | GGCCCAAACTCCATAGC | ES448350.1 | 202 | TAC | 50.4 | Perfect |
| Â | MESTSSR23R | CCGCCAATTCTAGACCAATG | Â | Â | Â | Â | Â |
6 | MESTSSR26F | CGTGATTACCTTCGGATTGG | ES448391.1 | 219 | AGCTGG | 57.9 | Perfect |
| Â | MESTSSR26R | CCAACCCAGTAGACCCAGTG | Â | Â | Â | Â | Â |
7 | MESTSSR27F | CCAACATTATCCGGAACACC | ES448394.1 | 266 | CGG | 54.8 | Perfect |
| Â | MESTSSR27R | GGTAAAGCCATCCGTTGC | Â | Â | Â | Â | Â |
8 | MESTSSR28F | GCCCAGTTTCCCACAGAA | ES448403.1 | 217 | ATA | 47.9 | Perfect |
| Â | MESTSSR28R | GGATGGTTTGTGCGTGC | Â | Â | Â | Â | Â |
9 | MESTSSR31F | CACCAATTAAAAGCGCAGTG | ES448813.1 | 204 | GA | 57.9 | Perfect |
| Â | MESTSSR31R | CTTTGTGGTTGGCTCGTG | Â | Â | Â | Â | Â |
10 | MESTSSR35F | CGTTTTCCGCTTCAGAGAG | ES448478.1 | 206 | AG | 54.8 | Perfect |
| Â | MESTSSR35R | GCCGATATCCTCCTTTCCTC | Â | Â | Â | Â | Â |
11 | MESTSSR37F | CAAAAGCGGTTTGGAATAGC | ES448476.1 | 245 | (CTTTC) CTCC(T) | 54.8 | interrupted |
| Â | MESTSSR37R | CCTCAACACAAAACCCACC | Â | Â | Â | Â | Â |
12 | MESTSSR40F | GAATCCTACAAGGGAGC | ES449069.1 | 215 | AAAAT | 52.8 | Perfect |
| Â | MESTSSR40R | CATACAAGGATGCCCACC | Â | Â | Â | Â | Â |
13 | MESTSSR41F | GGTCGACAAGAGGTAATC | ES449022.1 | 121 | AAAAG | 56.7 | Perfect |
| Â | MESTSSR41R | GAAGGCACCGAAGAGAAC | Â | Â | Â | Â | Â |
14 | MESTSSR42F | CAAGAGGTAATCCGTTC | ES448502.1 | 254 | AG | 54.8 | Perfect |
| Â | MESTSSR42R | CGTTGTTAGCAGGAGC | Â | Â | Â | Â | Â |
15 | MESTSSR46F | GCCCATGTTTGCGGAG | ES449184.1 | 200 | AG | 56.7 | Perfect |
| Â | MESTSSR46R | GGATTTTTCTGTCTGGGTG | Â | Â | Â | Â | Â |
16 | MESTSSR47F | GACTGCGGGAGAACAG | ES448510.1 | 220 | CTC | 54.8 | Perfect |
| Â | MESTSSR47R | GTTCACCGAGGCTGAGAG | Â | Â | Â | Â | Â |
17 | MESTSSR48F | GTTGTGGTGGTTGTTGC | ES448516.1 | 201 | TC | 56.7 | Perfect |
| Â | MESTSSR48R | CCTTCACTTTCTCGCC | Â | Â | Â | Â | Â |
18 | MESTSSR49F | CTTCGACGCCTTCTGCG | ES448598.1 | 184 | GAAGA | 56.7 | Perfect |
| Â | MESTSSR49R | GAGCGTCTCGAAGCAGTTG | Â | Â | Â | Â | Â |
19 | MESTSSR50F | GCCGGCATGTACGGATA | ES448967.1 | 235 | CCTAAC | 54.8 | Perfect |
| Â | MESTSSR50R | GTAAAAGTTTCGCCCCAGG | Â | Â | Â | Â | Â |
20 | MESTSSR51F | CCTAGGGTTTCCTTCGCTTC | ES448621.1 | 223 | GCG | 54.8 | Perfect |
| Â | MESTSSR51R | CGCTTAGGCTCCTTCCTC | Â | Â | Â | Â | Â |
21 | MESTSSR52F | CTTCGTTACGCTCGCTATG | ES448640.1 | 261 | TATTTT | 56.7 | Perfect |
| Â | MESTSSR52R | CCTTCTCTCAAGAATACTGG | Â | Â | Â | Â | Â |
22 | MESTSSR53F | GGCCAACATGTACGGATAG | ES449078.1 | 203 | CCTAAC | 56.7 | Perfect |
| Â | MESTSSR53R | CGCCAGGTACAACAAGAAG | Â | Â | Â | Â | Â |
23 | MESTSSR56F | CATTGCGTTCCTTGAG | ES448442.1 | 220 | ATCATG | 58.8 | Perfect |
| Â | MESTSSR56R | GGAGCCAAGACTCCTAAG | Â | Â | Â | Â | Â |
24 | MESTSSR59F | GAGCTCCGACGACCAC | ES448462.1 | 236 | TCATGA | 54.8 | Perfect |
| Â | MESTSSR59R | GCGTCTCGACGTGAGAAATAAC | Â | Â | Â | Â | Â |
25 | MESTSSR61F | CCATAGCCTCAACGTTTC | ES448534.1 | 239 | AAAAAC | 54.8 | Perfect |
| Â | MESTSSR61R | CGCTCACGTCCGTATC | Â | Â | Â | Â | Â |
26 | MESTSSR66F | GGAAAATTCATCCCCCAAGC | ES448761.1 | 258 | TTTTTG | 53.8 | Perfect |
| Â | MESTSSR66R | CGATGAGAAGCTCAAGGAG | Â | Â | Â | Â | Â |
27 | MESTSSR67F | GTGCTCGTAGCTTTGATGG | ES448763.1 | 215 | ATCGCC | 54.8 | Perfect |
| Â | MESTSSR67R | GCGAAGGAGAAGGAGGAGAG | Â | Â | Â | Â | Â |
28 | MESTSSR73F | CTCAAGCTATGCATCCAACGC | ES448909.1 | 237 | CT | 52.8 | Perfect |
| Â | MESTSSR73R | CCACTTCGAGAGCTTCG | Â | Â | Â | Â | Â |
29 | MESTSSR74F | CCATGGCTGAGCACGAG | ES448909.1 | 238 | GAA, GAG | 52.8 | compound |
| Â | MESTSSR74R | GAGCTCCAGTGTTCCTC | Â | Â | Â | Â | Â |
30 | MESTSSR76F | GATCCAGAACTCCCAAACC | ES448912.1 | 209 | CTCCGT | 50.4 | Perfect |
| Â | MESTSSR76R | GGTAATCCGAGTTCGAGACG | Â | Â | Â | Â | Â |
31 | MESTSSR77F | CCATAGCCTCAACGTTTC | ES448915.1 | 238 | AAAAAC | 52.8 | Perfect |
| Â | MESTSSR77R | CGCTCACGTCCGTATC | Â | Â | Â | Â | Â |
32 | MESTSSR78F | GCACTCTCAAACAAATCCTC | ES448921.1 | 242 | AAGTGG | 52.8 | Perfect |
| Â | MESTSSR78R | CGTTTGGAAACGGCTACTTC | Â | Â | Â | Â | Â |
33 | MESTSSR79F | CCCATAGCCTCAACGTTTC | ES448926.1 | 221 | AAAAAC | 45.9 | Perfect |
| Â | MESTSSR79R | CGACAACAACCGTCAAGTC | Â | Â | Â | Â | Â |
34 | MESTSSR85F | GTCATCTATGTCGGGTGGTC | ES448670.1 | 310 | ATACAT | 55.4 | Perfect |
| Â | MESTSSR85R | CATGGAGCGTTTGTTGTGTG | Â | Â | Â | Â | Â |
35 | MESTSSR99F | GGCCAACATGTACGGATAG | ES448967.1 | 203 | CCTAAC | 50.4 | Perfect |
| Â | MESTSSR99R | CGCCAGGTACAACAAGAAG | Â | Â | Â | Â | Â |
36 | MESTSSR108F | GGCTCTGAATGTCCGAGAAG | ES448289.1 | 246 | GAGTTG | 50.4 | Perfect |
| Â | MESTSSR108R | GGGTGGTAGATTTGGCAC | Â | Â | Â | Â | Â |
37 | MESTSSR109F | CTCACGTCCGTATCATCG | ES448314.1 | 244 | TTTGTT | 50.4 | Perfect |
| Â | MESTSSR109R | CCATTCCCATAGCCTCAAC | Â | Â | Â | Â | Â |
38 | MESTSSR111F | CATCTATGTCGGGTGGTCG | ES449122.1 | 299 | AAAT | 45.9 | Perfect |
| Â | MESTSSR111R | CTATGCACAACAGGCTGC | Â | Â | Â | Â | Â |
39 | MESTSSR113F | GCCTCCCATTATGCACTATG | ES449132.1 | 206 | AAAACA | 52.8 | Perfect |
| Â | MESTSSR113R | CGGATCTTCCAGGCTC | Â | Â | Â | Â | Â |
40 | MESTSSR115F | CAGGAATCAGAGCCAGAGC | ES448647.1 | 398 | AAAAAC | 53.8 | Perfect |
| Â | MESTSSR115R | CTGGACCATGTGGAAGC | Â | Â | Â | Â | Â |
41 | MESTSSR117F | CATTATCCGGAACACCAGACG | ES448396.1 | 247 | CGG | 53.8 | Perfect |
| Â | MESTSSR117R | GCTAAGAACCTCGCTCG | Â | Â | Â | Â | Â |
42 | MESTSSR121F | CACGTCCGTATCATCGG | ES449197.1 | 244 | TTTGTT | 52.8 | Perfect |
| Â | MESTSSR121R | CCATTCCCATAGCCTCAAC | Â | Â | Â | Â | Â |
43 | MESTSSR129F | GATTACTCCAACCAACTCC | ES449040.1 | 223 | AAAACC | 52.8 | Perfect |
| Â | MESTSSR129R | CAAGGGGGCTAGGAAG | Â | Â | Â | Â | Â |
44 | MESTSSR123F | CATCTATGTCGGGTGGTCG | ES448449.1 | 240 | CT | 52.8 | Perfect |
| Â | MESTSSR123R | GTGTTTGCTGGACTTTGC | Â | Â | Â | Â | Â |
45 | MESTSSR126F | CACCGATGAGCCCTGGTC | ES448693.1 | 200 | TTC | 52.8 | Perfect |
| Â | MESTSSR126R | GCACAATCCATCCCAAGTG | Â | Â | Â | Â | Â |
46 | MESTSSR127F | CCAACATTATCCGGAACACC | ES448594.1 | 285 | CGG | 52.8 | Perfect |
| Â | MESTSSR127R | CCTGGACGGAAGAAGTGG | Â | Â | Â | Â | Â |
47 | MESTSSR131F | CCTCATTGCGTTCCTTGAG | ES448442.1 | 225 | ATA, ATCATG | 54.1 | compound |
| Â | MESTSSR131R | CTGATTTGGGAGCCAAGAC | Â | Â | Â | Â | Â |
48 | MESTSSR132F | CTATGTCGGGTGGTCG | GT735086.1 | 473 | TTTTCC | 54.1 | Perfect |
| Â | MESTSSR132R | CATACCGTCGGAGATGC | Â | Â | Â | Â | Â |
49 | MESTSSR136F | CCATTCCCATAGCCTC | ES449178.1 | 244 | AAAAAC | 50.5 | Perfect |
| Â | MESTSSR136R | CGTCCGTATCATCGG | Â | Â | Â | Â | Â |
50 | MESTSSR134F | GGTTGTTGTCGAATCCG | ES448600.1 | 208 | TTTGTT | 55.4 | Perfect |
| Â | MESTSSR134R | GTACAAACCGAACGGGAAC | Â | Â | Â | Â | Â |
51 | MESTSSR135F | CCTCATTGCGTTCCTTG | ES448442.1 | 219 | ATCATG | 54.1 | Perfect |
| Â | MESTSSR135R | CCGGTGAGGTGATTGG | Â | Â | Â | Â | Â |