|
Sl no
|
Primer name
|
Primer sequence
|
GenBank-ID
|
Amplicon size
|
Repeat motif
|
Ta (0°C)
|
Repeat type
|
|---|
|
1
|
MESTSSR10F
|
CATTGCACATTGCAGGTAGC
|
GT629469.1
|
237
|
GTT
|
52.8
|
Perfect
|
| |
MESTSSR10R
|
CGGCCATCCAAAATGTTGTTC
| | | | | |
|
2
|
MESTSSR13F
|
TCTATCTCAACCGGAAGTCC
|
GT628644.1
|
230
|
(CAAAAG)G(AAAATA)
|
54.8
|
interrupted
|
| |
MESTSSR13R
|
CCAATTTGCTCGTCTTATGC
| | | | | |
|
3
|
MESTSSR14F
|
CGGCCACAGGTACTTTC
|
GT628768.1
|
202
|
TTGATT
|
50.4
|
Perfect
|
| |
MESTSSR14R
|
GGCAGCGATTTAGGATTGG
| | | | | |
|
4
|
MESTSSR20F
|
CGCAAGTGTCTCAACTG
|
GT629110.1
|
200
|
TGA
|
49.1
|
Perfect
|
| |
MESTSSR20R
|
GGAACGGATGGAGTAAG
| | | | | |
|
5
|
MESTSSR23F
|
GGCCCAAACTCCATAGC
|
ES448350.1
|
202
|
TAC
|
50.4
|
Perfect
|
| |
MESTSSR23R
|
CCGCCAATTCTAGACCAATG
| | | | | |
|
6
|
MESTSSR26F
|
CGTGATTACCTTCGGATTGG
|
ES448391.1
|
219
|
AGCTGG
|
57.9
|
Perfect
|
| |
MESTSSR26R
|
CCAACCCAGTAGACCCAGTG
| | | | | |
|
7
|
MESTSSR27F
|
CCAACATTATCCGGAACACC
|
ES448394.1
|
266
|
CGG
|
54.8
|
Perfect
|
| |
MESTSSR27R
|
GGTAAAGCCATCCGTTGC
| | | | | |
|
8
|
MESTSSR28F
|
GCCCAGTTTCCCACAGAA
|
ES448403.1
|
217
|
ATA
|
47.9
|
Perfect
|
| |
MESTSSR28R
|
GGATGGTTTGTGCGTGC
| | | | | |
|
9
|
MESTSSR31F
|
CACCAATTAAAAGCGCAGTG
|
ES448813.1
|
204
|
GA
|
57.9
|
Perfect
|
| |
MESTSSR31R
|
CTTTGTGGTTGGCTCGTG
| | | | | |
|
10
|
MESTSSR35F
|
CGTTTTCCGCTTCAGAGAG
|
ES448478.1
|
206
|
AG
|
54.8
|
Perfect
|
| |
MESTSSR35R
|
GCCGATATCCTCCTTTCCTC
| | | | | |
|
11
|
MESTSSR37F
|
CAAAAGCGGTTTGGAATAGC
|
ES448476.1
|
245
|
(CTTTC) CTCC(T)
|
54.8
|
interrupted
|
| |
MESTSSR37R
|
CCTCAACACAAAACCCACC
| | | | | |
|
12
|
MESTSSR40F
|
GAATCCTACAAGGGAGC
|
ES449069.1
|
215
|
AAAAT
|
52.8
|
Perfect
|
| |
MESTSSR40R
|
CATACAAGGATGCCCACC
| | | | | |
|
13
|
MESTSSR41F
|
GGTCGACAAGAGGTAATC
|
ES449022.1
|
121
|
AAAAG
|
56.7
|
Perfect
|
| |
MESTSSR41R
|
GAAGGCACCGAAGAGAAC
| | | | | |
|
14
|
MESTSSR42F
|
CAAGAGGTAATCCGTTC
|
ES448502.1
|
254
|
AG
|
54.8
|
Perfect
|
| |
MESTSSR42R
|
CGTTGTTAGCAGGAGC
| | | | | |
|
15
|
MESTSSR46F
|
GCCCATGTTTGCGGAG
|
ES449184.1
|
200
|
AG
|
56.7
|
Perfect
|
| |
MESTSSR46R
|
GGATTTTTCTGTCTGGGTG
| | | | | |
|
16
|
MESTSSR47F
|
GACTGCGGGAGAACAG
|
ES448510.1
|
220
|
CTC
|
54.8
|
Perfect
|
| |
MESTSSR47R
|
GTTCACCGAGGCTGAGAG
| | | | | |
|
17
|
MESTSSR48F
|
GTTGTGGTGGTTGTTGC
|
ES448516.1
|
201
|
TC
|
56.7
|
Perfect
|
| |
MESTSSR48R
|
CCTTCACTTTCTCGCC
| | | | | |
|
18
|
MESTSSR49F
|
CTTCGACGCCTTCTGCG
|
ES448598.1
|
184
|
GAAGA
|
56.7
|
Perfect
|
| |
MESTSSR49R
|
GAGCGTCTCGAAGCAGTTG
| | | | | |
|
19
|
MESTSSR50F
|
GCCGGCATGTACGGATA
|
ES448967.1
|
235
|
CCTAAC
|
54.8
|
Perfect
|
| |
MESTSSR50R
|
GTAAAAGTTTCGCCCCAGG
| | | | | |
|
20
|
MESTSSR51F
|
CCTAGGGTTTCCTTCGCTTC
|
ES448621.1
|
223
|
GCG
|
54.8
|
Perfect
|
| |
MESTSSR51R
|
CGCTTAGGCTCCTTCCTC
| | | | | |
|
21
|
MESTSSR52F
|
CTTCGTTACGCTCGCTATG
|
ES448640.1
|
261
|
TATTTT
|
56.7
|
Perfect
|
| |
MESTSSR52R
|
CCTTCTCTCAAGAATACTGG
| | | | | |
|
22
|
MESTSSR53F
|
GGCCAACATGTACGGATAG
|
ES449078.1
|
203
|
CCTAAC
|
56.7
|
Perfect
|
| |
MESTSSR53R
|
CGCCAGGTACAACAAGAAG
| | | | | |
|
23
|
MESTSSR56F
|
CATTGCGTTCCTTGAG
|
ES448442.1
|
220
|
ATCATG
|
58.8
|
Perfect
|
| |
MESTSSR56R
|
GGAGCCAAGACTCCTAAG
| | | | | |
|
24
|
MESTSSR59F
|
GAGCTCCGACGACCAC
|
ES448462.1
|
236
|
TCATGA
|
54.8
|
Perfect
|
| |
MESTSSR59R
|
GCGTCTCGACGTGAGAAATAAC
| | | | | |
|
25
|
MESTSSR61F
|
CCATAGCCTCAACGTTTC
|
ES448534.1
|
239
|
AAAAAC
|
54.8
|
Perfect
|
| |
MESTSSR61R
|
CGCTCACGTCCGTATC
| | | | | |
|
26
|
MESTSSR66F
|
GGAAAATTCATCCCCCAAGC
|
ES448761.1
|
258
|
TTTTTG
|
53.8
|
Perfect
|
| |
MESTSSR66R
|
CGATGAGAAGCTCAAGGAG
| | | | | |
|
27
|
MESTSSR67F
|
GTGCTCGTAGCTTTGATGG
|
ES448763.1
|
215
|
ATCGCC
|
54.8
|
Perfect
|
| |
MESTSSR67R
|
GCGAAGGAGAAGGAGGAGAG
| | | | | |
|
28
|
MESTSSR73F
|
CTCAAGCTATGCATCCAACGC
|
ES448909.1
|
237
|
CT
|
52.8
|
Perfect
|
| |
MESTSSR73R
|
CCACTTCGAGAGCTTCG
| | | | | |
|
29
|
MESTSSR74F
|
CCATGGCTGAGCACGAG
|
ES448909.1
|
238
|
GAA, GAG
|
52.8
|
compound
|
| |
MESTSSR74R
|
GAGCTCCAGTGTTCCTC
| | | | | |
|
30
|
MESTSSR76F
|
GATCCAGAACTCCCAAACC
|
ES448912.1
|
209
|
CTCCGT
|
50.4
|
Perfect
|
| |
MESTSSR76R
|
GGTAATCCGAGTTCGAGACG
| | | | | |
|
31
|
MESTSSR77F
|
CCATAGCCTCAACGTTTC
|
ES448915.1
|
238
|
AAAAAC
|
52.8
|
Perfect
|
| |
MESTSSR77R
|
CGCTCACGTCCGTATC
| | | | | |
|
32
|
MESTSSR78F
|
GCACTCTCAAACAAATCCTC
|
ES448921.1
|
242
|
AAGTGG
|
52.8
|
Perfect
|
| |
MESTSSR78R
|
CGTTTGGAAACGGCTACTTC
| | | | | |
|
33
|
MESTSSR79F
|
CCCATAGCCTCAACGTTTC
|
ES448926.1
|
221
|
AAAAAC
|
45.9
|
Perfect
|
| |
MESTSSR79R
|
CGACAACAACCGTCAAGTC
| | | | | |
|
34
|
MESTSSR85F
|
GTCATCTATGTCGGGTGGTC
|
ES448670.1
|
310
|
ATACAT
|
55.4
|
Perfect
|
| |
MESTSSR85R
|
CATGGAGCGTTTGTTGTGTG
| | | | | |
|
35
|
MESTSSR99F
|
GGCCAACATGTACGGATAG
|
ES448967.1
|
203
|
CCTAAC
|
50.4
|
Perfect
|
| |
MESTSSR99R
|
CGCCAGGTACAACAAGAAG
| | | | | |
|
36
|
MESTSSR108F
|
GGCTCTGAATGTCCGAGAAG
|
ES448289.1
|
246
|
GAGTTG
|
50.4
|
Perfect
|
| |
MESTSSR108R
|
GGGTGGTAGATTTGGCAC
| | | | | |
|
37
|
MESTSSR109F
|
CTCACGTCCGTATCATCG
|
ES448314.1
|
244
|
TTTGTT
|
50.4
|
Perfect
|
| |
MESTSSR109R
|
CCATTCCCATAGCCTCAAC
| | | | | |
|
38
|
MESTSSR111F
|
CATCTATGTCGGGTGGTCG
|
ES449122.1
|
299
|
AAAT
|
45.9
|
Perfect
|
| |
MESTSSR111R
|
CTATGCACAACAGGCTGC
| | | | | |
|
39
|
MESTSSR113F
|
GCCTCCCATTATGCACTATG
|
ES449132.1
|
206
|
AAAACA
|
52.8
|
Perfect
|
| |
MESTSSR113R
|
CGGATCTTCCAGGCTC
| | | | | |
|
40
|
MESTSSR115F
|
CAGGAATCAGAGCCAGAGC
|
ES448647.1
|
398
|
AAAAAC
|
53.8
|
Perfect
|
| |
MESTSSR115R
|
CTGGACCATGTGGAAGC
| | | | | |
|
41
|
MESTSSR117F
|
CATTATCCGGAACACCAGACG
|
ES448396.1
|
247
|
CGG
|
53.8
|
Perfect
|
| |
MESTSSR117R
|
GCTAAGAACCTCGCTCG
| | | | | |
|
42
|
MESTSSR121F
|
CACGTCCGTATCATCGG
|
ES449197.1
|
244
|
TTTGTT
|
52.8
|
Perfect
|
| |
MESTSSR121R
|
CCATTCCCATAGCCTCAAC
| | | | | |
|
43
|
MESTSSR129F
|
GATTACTCCAACCAACTCC
|
ES449040.1
|
223
|
AAAACC
|
52.8
|
Perfect
|
| |
MESTSSR129R
|
CAAGGGGGCTAGGAAG
| | | | | |
|
44
|
MESTSSR123F
|
CATCTATGTCGGGTGGTCG
|
ES448449.1
|
240
|
CT
|
52.8
|
Perfect
|
| |
MESTSSR123R
|
GTGTTTGCTGGACTTTGC
| | | | | |
|
45
|
MESTSSR126F
|
CACCGATGAGCCCTGGTC
|
ES448693.1
|
200
|
TTC
|
52.8
|
Perfect
|
| |
MESTSSR126R
|
GCACAATCCATCCCAAGTG
| | | | | |
|
46
|
MESTSSR127F
|
CCAACATTATCCGGAACACC
|
ES448594.1
|
285
|
CGG
|
52.8
|
Perfect
|
| |
MESTSSR127R
|
CCTGGACGGAAGAAGTGG
| | | | | |
|
47
|
MESTSSR131F
|
CCTCATTGCGTTCCTTGAG
|
ES448442.1
|
225
|
ATA, ATCATG
|
54.1
|
compound
|
| |
MESTSSR131R
|
CTGATTTGGGAGCCAAGAC
| | | | | |
|
48
|
MESTSSR132F
|
CTATGTCGGGTGGTCG
|
GT735086.1
|
473
|
TTTTCC
|
54.1
|
Perfect
|
| |
MESTSSR132R
|
CATACCGTCGGAGATGC
| | | | | |
|
49
|
MESTSSR136F
|
CCATTCCCATAGCCTC
|
ES449178.1
|
244
|
AAAAAC
|
50.5
|
Perfect
|
| |
MESTSSR136R
|
CGTCCGTATCATCGG
| | | | | |
|
50
|
MESTSSR134F
|
GGTTGTTGTCGAATCCG
|
ES448600.1
|
208
|
TTTGTT
|
55.4
|
Perfect
|
| |
MESTSSR134R
|
GTACAAACCGAACGGGAAC
| | | | | |
|
51
|
MESTSSR135F
|
CCTCATTGCGTTCCTTG
|
ES448442.1
|
219
|
ATCATG
|
54.1
|
Perfect
|
| |
MESTSSR135R
|
CCGGTGAGGTGATTGG
| | | | | |
