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Figure 3 | BMC Plant Biology

Figure 3

From: A high-throughput virus-induced gene silencing protocol identifies genes involved in multi-stress tolerance

Figure 3

Salinity tolerance of gene-silenced leaf disks. Three-week-old N. benthamiana plants were inoculated with TRV carrying inserts of a gene of interest and grown in the greenhouse along with TRV::GFP (vector control) and wild-type non-inoculated plants. Leaf disks were collected from these plants at 20 dpi. Leaf disks were incubated on CIM supplemented with either 100 or 200 mM NaCl for 20 days under the environmental conditions described in Figure 1. For non-stress, leaf disks were maintained on CIM without NaCl. Callus dry weight was measured at the end of the stress period. Each bar value represents mean ± sd (n = 12) of three independent experiments. Values significantly different from wild-type and vector control plants were indicated by ‘*’, and the rest were non-significant (Student’s t-test, P < 0.05).

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