Gene expression of CaACO (A), CaACS (B) and CaETR (C) isoforms during capsicum ripening as determined by qPCR. G, Green; B, Breaker; BR1, Breaker Red 1; BR2, Breaker Red 2; LR, Light Red; DR, Deep Red. Bars represent the mean ± SE of n = 3 biological replicates. The same letter indicates no difference between means as determined using the Least Significant Difference (P < 0.05). Gene expression was normalised relative to CaGAPdH expression according to the Methods. Note that the relative expression axis was set at a similar value for CaACO, CaACS and CaETR, respectively. For CaACO1, CaACO2, CaACO3, CaACO5 and CaACO6, inset figures are shown with respect to their relative expression values corresponding to the ripening stages (G, B, BR1, BR2, LR, DR) and the double slash on both DR stage bars of CaACO1 and CaACO3 indicate values higher than the maximum (A).