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Figure 5 | BMC Plant Biology

Figure 5

From: The M3 phosphorylation motif has been functionally conserved for intracellular trafficking of long-looped PIN-FORMEDs in the Arabidopsis root hair cell

Figure 5

M3 phosphorylation-defective PIN2 proteins could not be restored by auxin or MG132 in root hair cells. (A) M3 phosphorylation-defective PIN2 proteins were not restored by exogenous auxin in root hair cells. GFP signals of ProE7:PIN2:GFP, ProE7:3 m1-PIN2:GFP, and ProE7:M3-PIN2:GFP were visualized. Transgenic seedlings were treated with 5 μM NAA for 2 h and stained with FM4-64 (2 μM). Representative images are shown. Bar = 10 μm in all cases. (B) M3 phosphorylation-defective PIN2 proteins were not restored by MG132 treatment in root hair cells. Transgenic seedlings were treated with 25 μM MG132 for 2.5 h and stained with FM4-64. Representative images are shown. Bar = 10 μm in all cases.

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