Figure 5

Identification of CaMNUC32 isoforms by two-dimensional in-gel nuclease activity assay. Nuclease activities of total protein extracts (125 μg) subjected to 2D-nuclease activity assays (IEF in 12 cm strips). (CON) Total protein extract from cultured cells treated with control medium supplemented with DMSO during 30 days. (CPT) Total protein extract from cultured cells treated with 0.5 μM CPT during 30 days (CPT).