Figure 3

Characterization of CaMNUC32 nuclease activity. (A) Protein extracted from maize cultured cells (15 μg) treated with 0.5 μM CPT (+) or untreated (-) during 30 days were subjected to in-gel DNase activity assay under different conditions of pH and cationic requirements. The conditions of the nuclease assays are indicated: Ca²⁺, 1 mM CaCl₂; Mg²⁺, 1 mM MgCl₂; Zn²⁺, 5 mM ZnSO₄; EDTA, 1 mM EDTA. (B) Nuclease activity assays were performed in presence of calf thymus single stranded DNA (ssDNA) or salmon sperm double stranded DNA (dsDNA), in 1 mM CaCl₂, 1 mM MgCl₂ and pH 7.5.