Figure 5
Expression of candidate genes in PRC2 VIGS-treated floral organs. A. Expression of several floral organ identity genes in AqANS-silenced control sepals (C1-C4), and AqFIE- (F2-F6) and AqEMF2- (E1-E4s/p) treated first whorl organs. AqAG1 is up-regulated in all AqFIE- and AqEMF2-treated organs compared to the controls.AqAP3-3 also appears to be up-regulated in some of the sepals, particularly in AqEMF2 down-regulated first whorl organs, several of which were in fact sepal/petal chimeras (s/p). Expression of AqAP3-2 and AqFL1 is variable in mature sepals and is difficult to assess. AqAG2 and AqAP3-1 expression does not appear to be affected in silenced tissue. B. Expression of several floral organ identity genes in AqANS-silenced control petals (C1-C4), and AqFIE- (F2-F6) and AqEMF2- (E1-E4s/p) VIGS-treated petals. AqAG1 is up-regulated in all AqFIE- and AqEMF2-treated tissue compared to the controls. C and D. Quantitative Real Time PCR analysis of expression of AqAG1 and AqAG2 in AqFIE, AqEMF2, and AqANS control silenced tissue and wild type carpels. cDNA from two to four samples was pooled together prior to analysis. For each data point, three technical replicates were analyzed. AqIPP2 expression was used for normalization. C. Average fold change in the expression of AqAG1 in AqFIE, AqEMF2, and AqANS control silenced tissue normalized to wild type carpels with SD error bars. D. Average fold change in the expression of AqAG2 in AqFIE, AqEMF2, and AqANS control silenced tissue normalized to wild type carpels with SD error bars.