Figure 7

Immunofluorescence labelling for calcium-associated HG regions with antibody 2F4 in mature and ripe apple fruit. ‘Royal Gala’ (A, B) and ‘Scifresh’ (C, D) apple cortex tissue of mature fruit and ripe fruit. 2F4-labelling (green) was concentrated at tricellular junctions as indicated by the arrows. No labelling was detected in ripe ‘Royal Gala’ (B). Bar in (A) = 10 μm for all micrographs.