Figure 1

Expression of AtOCD in response to low water potential stress. A. QPCR analysis of transcript accumulation of AtOCD transcript levels after 10 or 96 h of low water potential stress. After 96 h of stress, seedlings were transferred back to the high water potential control (−0.25 MPa) for 10 or 96 h. Data are means ± SE (N = 5-6). B. Root versus shoot expression of AtOCD in control and −1.2 MPa treatments. Data are means ± SE (N = 5-6). C. Histochemical GUS staining of T₃ Homozygous AtOCDpromoter:GUS transgenic plants expressing GUS under control of the 1.5 kb promoter region of AtOCD. Seven day old seedlings were transferred to −1.2 MPa stress treatment up to 96 h. Three independent transgenic lines were analyzed and representative images are shown. Scale bar in each panel equals 1 mm. D. GUS staining of floral organs of AtOCDpro::GUS plants under normal growing conditions or after water withholding. Scale bar in each panel equals 1 mm.