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Figure 4 | BMC Plant Biology

Figure 4

From: Genome-wide identification of Thellungiella salsuginea microRNAs with putative roles in the salt stress response

Figure 4

Relative expression of miRNAs as determined by stem-loop qRT-PCR. Total RNA isolated from control seedlings and those treated with 200 mM NaCl. The reverse transcription reaction was carried out using stem-loop primers (Additional file 9: Table S9) of these miRNAs. The level of expression was normalized to that of U6. The normalized miRNA levels in the control were arbitrarily set to 1. Error bars indicate ± SE obtained from three biological repeats. Student’s T-test was performed to analyze the changes in the gene expression after treated with NaCl. **denotes the p value < 0.01 and *denotes the p value < 0.05.

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