Figure 5From: Spliceosomal introns in the 5′ untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expressionQuantitative GUS assay of the AthBTL1 and AthBTL4 promoter lines. The mean values and standard deviations of GUS activity were calculated from three replicates and represented as nanomoles of 4-methyl umbelliferone per milligram protein per minute, as described in Methods. The gray bars represent the expression of the pAthBTL1: GUS and pAthBTL4: GUS fusions harbouring the 5′UTR intron whereas empty bars represent the expression of the intronless promoter: GUS fusions.Back to article page