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Figure 2 | BMC Plant Biology

Figure 2

From: Spliceosomal introns in the 5′ untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expression

Figure 2

IMEter score and intron size of BTL genes containing a 5′UTR-intron. A phylogenetic tree of the 81 retrieved BTL sequences is display; it was generated as described in Methods. The bootstrap values of 1000 replicates are shown at the branches. The intron size scale goes from 1 to 2000 nucleotides. Introns longer than 2000 nucleotides have the maximum value (zma|GRMZM2G417125, 3919 nt; zma|GRMZM2G176028, 6571 nt; cat|Cucsa.14251, 2149 nt; vvi|GSVIVG01037379001, 3518 nt, and egr|Eucgr.K01683, 3411 nt). The average size of introns in groups A and B was calculated after eliminating the extreme values, resulting in 1,133+/-801 nucleotides for group A and 578+/-444 for group B; the average sizes in monocots and eudicots were similar when measured independently. IMEter v2.0 scores: moderate enhancement, between 10 and 20, and strong enhancement, more than 20.

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