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Figure 1 | BMC Plant Biology

Figure 1

From: Spliceosomal introns in the 5′ untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expression

Figure 1

General features of BTLs . a. A schematic representation of a canonical Rabring7/BCA2/BTL E3 ligase showing the relative position of the BZF and RING-H2 domains. A sequence LOGO comparison between the two RING-H2 domains is shown; LOGOs were generated from the collected Rabring7/BCA2/BTLs sequences and from a previous analysis of the E3 ATLs. An alignment of five representative BTLs with the BZF and the RING-H2 regions displayed below. ClustalX was used for sequence alignment and a default color code was applied. The arrowheads point to the residues involved in zinc ligation in the RING-H2 domain and the conserved cysteines in the BZF domain. b. Donor and acceptor sites of 5′UTR introns from 63 BTLs. Twenty nucleotides of exon and intron sequences at the donor site were used to obtain the LOGO, as described in Methods.

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